PDF(Pubmed)
Abstract：
Despite the many beneficial properties of legume plants, their use in diets for poultry is limited by the presence of antinutritional factors. The aim of the study was to determine the activity of DT-diaphorase, ethoxycoumarin O-deethylase, and catalase, and the concentration of malondialdehyde in liver tissue, as well as the activity of SOD and CAT in the serum of Hy-line Brown hens fed a diet supplemented with various doses of Lupinus angustifolius seeds. The results indicate that the use of large amounts of lupin in the diet resulted in an increase in MDA concentration in the liver and the lipid vacuolization of hepatocytes. A significant increase in DTD activity was observed in chickens receiving 15% lupin. Regardless of lupin dose, no increase in SOD activity was observed in chicken serum after 33 days of the experiment. From the 66th day of the experiment, an increase in catalase activity in the serum of laying hens was observed, while low activity of this enzyme was found in the liver. It can be concluded that the short-term use of lupin in the diet of laying hens does not affect the activity of antioxidant enzymes and, therefore, does not affect the oxidative-antioxidant balance of their body.
摘要：
尽管豆科植物有许多有益的特性，抗营养因子的存在限制了它们在家禽饮食中的使用。这项研究的目的是确定DT-心肌黄递酶的活性，乙氧基香豆素O-脱乙基酶，和过氧化氢酶,肝脏组织中丙二醛的浓度，以及饲喂添加各种剂量羽扇豆种子的Hy系棕色母鸡血清中SOD和CAT的活性。结果表明，在饮食中使用大量羽扇豆导致肝脏中MDA浓度增加和肝细胞的脂质空泡化。在接受15%羽扇豆的鸡中观察到DTD活性的显著增加。不管羽扇豆的剂量，实验33天后，鸡血清中SOD活性没有增加。从实验的第66天开始，观察到产蛋鸡血清中过氧化氢酶活性增加，而在肝脏中发现了这种酶的低活性。可以得出结论，在蛋鸡日粮中短期使用羽扇豆并不影响抗氧化酶的活性，因此,不会影响他们身体的氧化-抗氧化剂平衡。
