PDF(Pubmed)
Abstract：
Plant waste materials are important sources of bioactive compounds with remarkable health-promoting benefits. In particular, industrial by-products such as mango peels are sustainable sources of bioactive substances, with antioxidant, enzymatic, and antimicrobial activity. Appropriate processing is essential to obtain highly bioactive compounds for further use in generating value-added products for the food industry. The objective of the study was to investigate and compare the biological activity of compounds from fresh and dried mango peels obtained by different conventional methods and unconventional extraction methods using supercritical fluids (SFE). The highest total phenolic content (25.0 mg GAE/g DW) and the total content of eight phenolic compounds (829.92 µg/g DW) determined by LC-MS/MS were detected in dried mango peel extract obtained by the Soxhlet process (SE). SFE gave the highest content of proanthocyanidins (0.4 mg PAC/g DW). The ethanolic ultrasonic process (UAE) provided the highest antioxidant activity of the product (82.4%) using DPPH radical scavenging activity and total protein content (2.95 mg protein/g DW). Overall, the dried mango peels were richer in bioactive compounds (caffeic acid, chlorogenic acid, gallic acid, catechin, and hesperidin/neohesperidin), indicating successful preservation during air drying. Furthermore, outstanding polyphenol oxidase, superoxide dismutase (SOD), and lipase activities were detected in mango peel extracts. This is the first study in which remarkable antibacterial activities against the growth of Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive bacteria (Bacillus cereus and Staphylococcus aureus) were evaluated by determining the microbial growth inhibition rate after 12 and 24 h incubation periods for mango peel extracts obtained by different methods. Ethanolic SE and UAE extracts from dried mango peels resulted in the lowest minimum inhibitory concentrations (MIC90) for all bacterial species tested. Mango peels are remarkable waste products that could contribute to the sustainable development of exceptional products with high-added value for various applications, especially as dietary supplements.
摘要：
植物废料是具有显著促进健康益处的生物活性化合物的重要来源。特别是,芒果果皮等工业副产品是生物活性物质的可持续来源，抗氧化剂，酶，和抗菌活性。适当的加工是必要的，以获得高生物活性的化合物，进一步用于产生食品工业的增值产品。研究的目的是研究和比较通过不同的常规方法和使用超临界流体（SFE）的非常规提取方法获得的新鲜和干燥芒果皮中化合物的生物活性。通过索氏工艺（SE）获得的干燥芒果皮提取物中检测到最高的总酚含量（25.0mgGAE/gDW）和通过LC-MS/MS测定的八种酚类化合物的总含量（829.92µg/gDW）。SFE的原花色素含量最高（0.4mgPAC/gDW）。乙醇超声工艺（UAE）使用DPPH自由基清除活性和总蛋白质含量（2.95mg蛋白质/gDW）提供了产品的最高抗氧化活性（82.4％）。总的来说,干芒果皮中富含生物活性化合物(咖啡酸，绿原酸,没食子酸,儿茶素,和橙皮苷/新橙皮苷)，表明在空气干燥过程中成功保存。此外，杰出的多酚氧化酶，超氧化物歧化酶（SOD），并检测了芒果皮提取物中的脂肪酶活性。这是第一项研究，其中通过测定12和24小时孵育期后的微生物生长抑制率来评估对革兰氏阴性菌（大肠杆菌和铜绿假单胞菌）和革兰氏阳性菌（蜡状芽孢杆菌和金黄色葡萄球菌）生长的显着抗菌活性。通过不同方法获得的芒果皮提取物。来自干燥芒果果皮的乙醇SE和UAE提取物导致所有测试的细菌物种的最低最低抑制浓度（MIC90）。芒果果皮是显着的废物，可以促进各种应用的高附加值的特殊产品的可持续发展，尤其是作为膳食补充剂。
