Abstract:
In a past study, the team used specific-locus amplified fragment sequencing (SLAF sequencing) to detect single-nucleotide polymorphisms (SNPs) contributing to the differences in lambing numbers in Xinjiang sheep. This study verified the correlation between the COIL gene and lambing number characters in sheep and explored its possible mechanism of action. In this study, three SNPs in the COIL gene, namely COILSNP1 (rs7321466), COILSNP2 (rs7314134), and COILSNP3 (rs7321563), were explored in terms of their possible mechanism of action. A tissue expression profiling analysis revealed that the COIL gene was significantly more expressed in the uterus and ovaries than in other tissues (p < 0.05), whereas an association analysis revealed that the number of lambs born was significantly different among individuals with different genotypes of this COILSNP1 (p < 0.05). The Cell Counting Kit-8(CCK-8) revealed that the overexpression of the COIL gene significantly increased the proliferation of mouse ovarian fibroblasts and sheep fibroblasts (p < 0.05). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) revealed that the overexpression of the COIL gene significantly increased the activity of sheep fibroblasts (p < 0.01) and mouse ovarian fibroblasts (p < 0.05). The overexpression of the COIL gene affected the biogenesis pathway of spliceosomal U snRNPs by validating protein network connections. This activity affects ovulation, embryonic development, and changes in lambing size in sheep.
摘要:
在过去的研究中,研究小组使用特定基因座扩增片段测序(SLAF测序)来检测导致新疆绵羊羔羊数量差异的单核苷酸多态性(SNPs).本研究验证了COIL基因与绵羊羔羊数性状的相关性,并探讨了其可能的作用机制。在这项研究中,COIL基因中的三个SNP,即COILSNP1(RS7321466),COILSNP2(RS7314134),和COILSNP3(RS7321563),从它们可能的作用机制方面进行了探讨。组织表达谱分析显示,COIL基因在子宫和卵巢中的表达明显高于其他组织(p<0.05),而关联分析显示,在具有该COILSNP1的不同基因型的个体中,出生的羔羊数量存在显着差异(p<0.05)。细胞计数试剂盒-8(CCK-8)显示,COIL基因的过表达显着增加了小鼠卵巢成纤维细胞和绵羊成纤维细胞的增殖(p<0.05)。3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物(MTT)显示COIL基因的过表达显著提高了绵羊成纤维细胞(p<0.01)和小鼠卵巢成纤维细胞(p<0.05)的活性。COIL基因的过表达通过验证蛋白质网络连接来影响剪接体UsnRNPs的生物发生途径。这种活动会影响排卵,胚胎发育,和绵羊羔羊大小的变化。