PDF(Pubmed)
Abstract：
Semen proteins play an important role in male reproductive performance and sperm fertilization ability and can be used as potential biomarkers to evaluate male fertility. The role of cysteine-rich secretory protein 3 (CRISP3) in male reproduction remains unknown. This study aimed to investigate the role of CRISP3 in the reproductive performance of boars. Our results showed that the CRISP3 protein content was significantly and positively correlated with boar fertility, sow delivery rate, and litter size. CRISP3 is highly expressed in the bulbourethral gland of adult boars and is enriched in the seminal plasma. It is localized in the post-acrosomal region of the sperm head and migrates to the anterior end of the tail after capacitation. The CRISP3 recombinant protein did not affect sperm motility and cleavage rate, but it significantly downregulated the mRNA expression of inflammatory factors IL-α, IL-1β, and IL-6 and the protein expression of IL-α and IL-6 in lipopolysaccharide (LPS)-induced RAW264.7 cells, indicating that CRISP3 has an immunomodulatory function. In conclusion, our study suggests that semen CRISP3 protein levels positively correlate with reproductive performance, which may be achieved by regulating immune responses in the female reproductive tract.
摘要：
精液蛋白在男性生殖性能和精子受精能力中起重要作用,可作为评价男性生育力的潜在生物标志物。富含半胱氨酸的分泌蛋白3（CRISP3）在男性生殖中的作用尚不清楚。本研究旨在探讨CRISP3在公猪繁殖性能中的作用。结果表明，CRISP3蛋白含量与公猪繁殖力呈显著正相关，母猪交货率，和垃圾大小。CRISP3在成年公猪的尿道球腺中高表达，并在精浆中富集。它位于精子头部的顶体后区域，并在获能后迁移到尾巴的前端。CRISP3重组蛋白不影响精子活力和卵裂率，但显著下调炎症因子IL-α的mRNA表达,IL-1β,脂多糖(LPS)诱导的RAW264.7细胞中IL-6、IL-α和IL-6的蛋白表达,表明CRISP3具有免疫调节功能。总之,我们的研究表明，精液CRISP3蛋白水平与生殖性能呈正相关，这可以通过调节女性生殖道的免疫反应来实现。
