PDF(Pubmed)
Abstract：
The aim of this study was to investigate the effects of aerobic treadmill training regimen of four weeks duration on oxidative stress parameters, metabolic enzymes, and histomorphometric changes in the colon of hyperhomocysteinemic rats. Male Wistar albino rats were divided into four groups (n = 10, per group): C, 0.9% NaCl 0.2 mL/day subcutaneous injection (s.c.) 2x/day; H, homocysteine 0.45 µmol/g b.w./day s.c. 2x/day; CPA, saline (0.9% NaCl 0.2 mL/day s.c. 2x/day) and an aerobic treadmill training program; and HPA, homocysteine (0.45 µmol/g b.w./day s.c. 2x/day) and an aerobic treadmill training program. The HPA group had an increased level of malondialdehyde (5.568 ± 0.872 μmol/mg protein, p = 0.0128 vs. CPA (3.080 ± 0.887 μmol/mg protein)), catalase activity (3.195 ± 0.533 U/mg protein, p < 0.0001 vs. C (1.467 ± 0.501 U/mg protein), p = 0.0012 vs. H (1.955 ± 0.293 U/mg protein), and p = 0.0003 vs. CPA (1.789 ± 0.256 U/mg protein)), and total superoxide dismutase activity (9.857 ± 1.566 U/mg protein, p < 0.0001 vs. C (6.738 ± 0.339 U/mg protein), p < 0.0001 vs. H (6.015 ± 0.424 U/mg protein), and p < 0.0001 vs. CPA (5.172 ± 0.284 U/mg protein)) were detected in the rat colon. In the HPA group, higher activities of lactate dehydrogenase (2.675 ± 1.364 mU/mg protein) were detected in comparison to the CPA group (1.198 ± 0.217 mU/mg protein, p = 0.0234) and higher activities of malate dehydrogenase (9.962 (5.752-10.220) mU/mg protein) were detected in comparison to the CPA group (4.727 (4.562-5.299) mU/mg protein, p = 0.0385). Subchronic treadmill training in the rats with hyperhomocysteinemia triggers the colon tissue antioxidant response (by increasing the activities of superoxide dismutase and catalase) and elicits an increase in metabolic enzyme activities (lactate dehydrogenase and malate dehydrogenase). This study offers a comprehensive assessment of the effects of aerobic exercise on colonic tissues in a rat model of hyperhomocysteinemia, evaluating a range of biological indicators including antioxidant enzyme activity, metabolic enzyme activity, and morphometric parameters, which suggested that exercise may confer protective effects at both the physiological and morphological levels.
摘要：
这项研究的目的是研究为期四周的有氧跑步机训练方案对氧化应激参数的影响，代谢酶，高同型半胱氨酸血症大鼠结肠的组织形态学变化。雄性Wistar白化病大鼠分为四组（每组10只）：C，0.9%NaCl0.2mL/天皮下注射(s.c.)2x/天;H,高半胱氨酸0.45µmol/gb.w./天s.c.2x/天；CPA，生理盐水（0.9%NaCl0.2mL/天，皮下2x/天）和有氧跑步机训练计划；和HPA，同型半胱氨酸（0.45µmol/gb.w./days.c.2x/day）和有氧跑步机训练计划。HPA组丙二醛水平升高(5.568±0.872μmol/mg蛋白,p=0.0128vs.CPA（3.080±0.887μmol/mg蛋白），过氧化氢酶活性(3.195±0.533U/mg蛋白，p<0.0001vs.C（1.467±0.501U/mg蛋白质），p=0.0012vs.H（1.955±0.293U/mg蛋白），和p=0.0003vs.CPA（1.789±0.256U/mg蛋白质），和总超氧化物歧化酶活性（9.857±1.566U/mg蛋白，p<0.0001vs.C（6.738±0.339U/mg蛋白），p<0.0001vs.H（6.015±0.424U/mg蛋白），和p<0.0001vs.在大鼠结肠中检测到CPA（5.172±0.284U/mg蛋白）。在HPA组中，与CPA组相比，检测到更高的乳酸脱氢酶活性（2.675±1.364mU/mg蛋白）（1.198±0.217mU/mg蛋白，与CPA组相比，p=0.0234）和更高的苹果酸脱氢酶活性（9.962（5.752-10.220）mU/mg蛋白）（4.727（4.562-5.299）mU/mg蛋白，p=0.0385)。高同型半胱氨酸血症大鼠的亚慢性跑步机训练会触发结肠组织抗氧化反应（通过增加超氧化物歧化酶和过氧化氢酶的活性），并引起代谢酶活性（乳酸脱氢酶和苹果酸脱氢酶）的增加。本研究全面评估了有氧运动对高同型半胱氨酸血症大鼠模型结肠组织的影响,评估一系列生物指标，包括抗氧化酶活性，代谢酶活性，和形态参数，这表明运动可以在生理和形态水平上赋予保护作用。
