PDF(Pubmed)
Abstract：
BACKGROUND: Deficient DNA mismatch repair (MMR) can cause microsatellite instability (MSI) and is more common in colorectal cancer (CRC) patients. Understanding the carcinogenic mechanism of bacteria and their impact on cancer cells is crucial. Bacteroides fragilis (B. fragilis) has been identified as a potential promoter of tumorigenesis through the alteration of signaling pathways. This study aims to assess the expression levels of msh2, msh6, mlh1, and the relative frequency of B. fragilis in biopsy samples from CRC patients.
METHODS: Based on the sequence of mlh1, msh2, and msh6 genes, B. fragilis specific 16srRNA and bacterial universal 16srRNA specific primers were selected, and the expression levels of the target genes were analyzed using the Real-Time PCR method.
RESULTS: Significant increases in the expression levels of mlh1, msh2, and msh6 genes were observed in the cancer group. Additionally, the expression of these MMR genes showed a significant elevation in samples positive for B. fragilis presence. The relative frequency of B. fragilis in the cancer group demonstrated a significant rise compared to the control group.
CONCLUSIONS: The findings suggest a potential correlation between the abundance of B. fragilis and alterations in the expression of MMR genes. Since these genes can play a role in modifying colon cancer, investigating microbial characteristics and gene expression changes in CRC could offer a viable solution for CRC diagnosis.
METHODS: Based on the sequence of mlh1, msh2, and msh6 genes, B. fragilis specific 16srRNA and bacterial universal 16srRNA specific primers were selected, and the expression levels of the target genes were analyzed using the Real-Time PCR method.
RESULTS: Significant increases in the expression levels of mlh1, msh2, and msh6 genes were observed in the cancer group. Additionally, the expression of these MMR genes showed a significant elevation in samples positive for B. fragilis presence. The relative frequency of B. fragilis in the cancer group demonstrated a significant rise compared to the control group.
CONCLUSIONS: The findings suggest a potential correlation between the abundance of B. fragilis and alterations in the expression of MMR genes. Since these genes can play a role in modifying colon cancer, investigating microbial characteristics and gene expression changes in CRC could offer a viable solution for CRC diagnosis.
摘要：
背景：DNA错配修复（MMR）缺陷会导致微卫星不稳定（MSI），并且在结直肠癌（CRC）患者中更为常见。了解细菌的致癌机制及其对癌细胞的影响至关重要。脆弱拟杆菌(B.fragilis）已通过信号通路的改变被确定为肿瘤发生的潜在启动子。本研究旨在评估CRC患者活检样品中msh2，msh6，mlh1的表达水平和脆弱芽孢杆菌的相对频率。
方法：基于mlh1、msh2和msh6基因的序列，选择脆弱芽孢杆菌特异性16srRNA和细菌通用16srRNA特异性引物，并使用Real-TimePCR方法分析目标基因的表达水平。
结果：在癌症组中观察到mlh1，msh2和msh6基因的表达水平显着增加。此外,这些MMR基因的表达在对脆弱芽孢杆菌存在呈阳性的样品中显示出显著升高。与对照组相比，癌症组中脆弱芽孢杆菌的相对频率显着上升。
结论：研究结果表明，脆弱芽孢杆菌的丰度与MMR基因表达的改变之间存在潜在的相关性。因为这些基因可以在改变结肠癌中发挥作用，研究CRC的微生物特征和基因表达变化可以为CRC诊断提供可行的解决方案。
方法：基于mlh1、msh2和msh6基因的序列，选择脆弱芽孢杆菌特异性16srRNA和细菌通用16srRNA特异性引物，并使用Real-TimePCR方法分析目标基因的表达水平。
结果：在癌症组中观察到mlh1，msh2和msh6基因的表达水平显着增加。此外,这些MMR基因的表达在对脆弱芽孢杆菌存在呈阳性的样品中显示出显著升高。与对照组相比，癌症组中脆弱芽孢杆菌的相对频率显着上升。
结论：研究结果表明，脆弱芽孢杆菌的丰度与MMR基因表达的改变之间存在潜在的相关性。因为这些基因可以在改变结肠癌中发挥作用，研究CRC的微生物特征和基因表达变化可以为CRC诊断提供可行的解决方案。
