METHODS: Based on the sequence of mlh1, msh2, and msh6 genes, B. fragilis specific 16srRNA and bacterial universal 16srRNA specific primers were selected, and the expression levels of the target genes were analyzed using the Real-Time PCR method.
RESULTS: Significant increases in the expression levels of mlh1, msh2, and msh6 genes were observed in the cancer group. Additionally, the expression of these MMR genes showed a significant elevation in samples positive for B. fragilis presence. The relative frequency of B. fragilis in the cancer group demonstrated a significant rise compared to the control group.
CONCLUSIONS: The findings suggest a potential correlation between the abundance of B. fragilis and alterations in the expression of MMR genes. Since these genes can play a role in modifying colon cancer, investigating microbial characteristics and gene expression changes in CRC could offer a viable solution for CRC diagnosis.
方法:基于mlh1、msh2和msh6基因的序列,选择脆弱芽孢杆菌特异性16srRNA和细菌通用16srRNA特异性引物,并使用Real-TimePCR方法分析目标基因的表达水平。
结果:在癌症组中观察到mlh1,msh2和msh6基因的表达水平显着增加。此外,这些MMR基因的表达在对脆弱芽孢杆菌存在呈阳性的样品中显示出显著升高。与对照组相比,癌症组中脆弱芽孢杆菌的相对频率显着上升。
结论:研究结果表明,脆弱芽孢杆菌的丰度与MMR基因表达的改变之间存在潜在的相关性。因为这些基因可以在改变结肠癌中发挥作用,研究CRC的微生物特征和基因表达变化可以为CRC诊断提供可行的解决方案。