Abstract：
Corydalis Rhizoma (CR, Yanhusuo in Chinese) has been widely used as an analgesic in herbal medicine and functional food. Cases of fungal and mycotoxin contamination in CR have been reported. In this study, the composition and diversity of fungal microbiome in CR samples from four herbal markets and two processing methods were investigated by DNA metabarcoding. Variations of the fungal microbiome in CR during cold and conventional storage were monitored. Results showed that Aspergillus was the dominant genus and saprotroph was the dominant trophic mode. Six potential toxigenic fungi, namely, Aspergillus fumigatus, Aspergillus ostianus, Aspergillus terreus, Penicillium citrinum, Penicillium oxalicum, and Trichothecium roseum, were detected. Differences in fungal composition and diversity among various groups based on collection areas and processing methods were also observed. Moreover, the relative abundance of dominant genera in CR samples stored at different temperatures was significantly different and changed with storage time. This study is the first to reveal the influence of collection areas, processing methods, and storage conditions on the fungal microbiome in CR, which was expected to provide a basis for control strategies of fungal contamination in the industrial chain of CR.
摘要：
延胡索（CR，中国的延胡索）已被广泛用作草药和功能食品中的镇痛药。已经报道了CR中真菌和霉菌毒素污染的病例。在这项研究中,通过DNA元编码研究了来自四个草药市场和两种加工方法的CR样品中真菌微生物组的组成和多样性。监测低温和常规储存期间CR中真菌微生物组的变化。结果表明，曲霉属为优势属，腐生菌为优势营养模式。六种潜在的产毒真菌，即,烟曲霉,曲霉,土曲霉,柑橘青霉,草酸青霉,和玫瑰三毛,被检测到。还观察到基于收集区域和处理方法的各组之间的真菌组成和多样性的差异。此外，在不同温度下储存的CR样品中，优势属的相对丰度存在显着差异，并随储存时间而变化。本研究首次揭示了收集区域的影响，处理方法，和CR中真菌微生物组的储存条件，有望为CR产业链中真菌污染的控制策略提供依据。
