Abstract：
To explore the potential mechanism of action of Tegillarca granosa polysaccharide (TGP) in treating nonalcoholic fatty liver disease (NAFLD), the study conducts in vivo experiments using male C57BL/6 mice fed a high-fat diet while administering TGP for 16 weeks. The study measures body weight, liver weight, serum biochemical markers, pathological histology, liver lipid accumulation, oxidative stress and inflammation-related factors, lipid synthesis and metabolism-related gene and protein expression, and the composition and abundance of intestinal flora. Additionally, short-chain fatty acid (SCFAs) content and the correlation between intestinal flora and environmental factors are measured. The results show that TGP effectively reduces excessive hepatic lipid accumulation, dyslipidemia, abnormal liver function, and steatosis in the mice with NAFLD. Moreover, TGP effectively regulates intestinal flora disorder, increases the diversity of intestinal flora, and affects the relative abundance of specific bacteria while also increasing the content of SCFAs. These findings provide a basis for exploring the regulatory effect of T. granosa polysaccharide on NAFLD based on intestinal flora and highlight its potential as a natural liver nutraceutical.
摘要：
探讨地瓜多糖(TGP)治疗非酒精性脂肪性肝病(NAFLD)的潜在作用机制。该研究使用饲喂高脂肪饮食的雄性C57BL/6小鼠进行体内实验，同时给予TGP16周。这项研究测量了体重，肝脏重量,血清生化标志物,病理组织学,肝脏脂质积累，氧化应激和炎症相关因素，脂质合成和代谢相关基因和蛋白质表达，以及肠道菌群的组成和丰度。此外,短链脂肪酸(SCFAs)含量及肠道菌群与环境因素的相关性。结果表明，TGP有效减少肝脏脂质过度积累，血脂异常,肝功能异常,NAFLD小鼠的脂肪变性。此外，TGP有效调节肠道菌群紊乱，增加肠道菌群的多样性，并影响特定细菌的相对丰度，同时也增加SCFA的含量。这些发现为探索基于肠道菌群的颗粒多糖对NAFLD的调节作用提供了基础，并突出了其作为天然肝脏营养保健品的潜力。
