Abstract：
Fish are ectotherms and many have an external reproductive mode. An environmental factor which triggers fish reproductive activity in fish is water temperature. However, climate change is causing increasingly frequent events in which the water temperature varies rapidly; as a result, both in hatchery and in natural conditions, fish sperm are exposed to varying environmental temperatures during their journey toward the egg. This study was based on two experiments: The first experiment was designed to determine how storage at 4 °C for four days affected the sperm functions of Atlantic salmon (Salmo salar) sperm collected by either abdominal massage (stripping/Pure) or testicular dissection (testicular macerate/Macerated). Further, computer-assisted semen analysis (CASA) was used to compare sperm velocity parameters (VCL, VSL, and VAP) and progressivity (STR, LIN, and WOB) after motility activation at different temperatures (8 and 16 °C) of sperm collected by both methods (Pure vs Macerated). The results show that spermatozoa from Macerated samples maintained a higher sperm function when stored at 4 °C for 4 days compared to Pure sperm samples. In the second experiment, CASA determined that all parameters for sperm velocity (VCL, VSL, and VAP) and progressivity (STR (50%/55%), LIN (25%-32%), and WOB (51%-57%) were affected by activation temperature (P < 0.05) and that the motility patterns after activation at 16 °C (P < 0.05), specifically the LIN or STR swimming trajectories of the sperm differed between the two groups. In conclusion, the sperm quality of testicular Macerate was superior to that of Pure sperm abdominal mass, based on the higher quality of various sperm functions during short-term storage. Moreover, there was a significant effect of the temperature of the activation medium on sperm speed and progressivity (motility pattern) in the collected samples of testicular macerate. The sensitivity of Salmo salar spermatozoa to elevated temperature varies markedly between collection methods (Pure and Macerated).
摘要：
鱼是异温动物，许多都有外部繁殖模式。触发鱼类繁殖活动的环境因素是水温。然而,气候变化导致水温快速变化的事件日益频繁；结果，在孵化场和自然条件下，鱼的精子在走向卵子的过程中暴露在不同的环境温度下。这项研究基于两个实验：第一个实验旨在确定在4°C下储存四天如何影响通过腹部按摩（剥离/纯）或睾丸解剖（睾丸浸软/浸软）收集的大西洋鲑鱼（Salmosalar）精子的精子功能。Further,计算机辅助精液分析(CASA)用于比较精子速度参数(VCL，VSL,和VAP)和累进性(STR，LIN,和WOB）在通过两种方法收集的精子在不同温度（8和16°C）下的运动性激活后（PurevsMacerated）。结果显示,与纯精子样品相比,来自经浸渍的样品的精子在4°C下储存4天时保持较高的精子功能。在第二个实验中,CASA确定精子速度的所有参数(VCL，VSL,和VAP)和累进性(STR(50%/55%)，LIN（25%-32%），和WOB（51％-57％）受活化温度的影响（P<0.05），并且在16°C活化后的运动模式（P<0.05），具体来说，两组之间精子的LIN或STR游泳轨迹不同。总之,睾丸的精子质量优于纯精子腹部质量，基于短期储存过程中各种精子功能的较高质量。此外，在收集的睾丸浸膏样品中，激活介质的温度对精子速度和进展性（运动性模式）有显著影响。Salmosalar精子对升高的温度的敏感性在收集方法（Pure和Macerated）之间明显不同。
