Abstract：
OBJECTIVE: Histone-deacetylases (HDACs) are epigenetic modulators involved in the control of gene expression. No data are available on the expression or subcellular localization of HDACs in salivary glands. The present study aims to examine the subcellular distribution of HDACs in salivary glands during postnatal development.
METHODS: The major salivary glands of C57/BL6 mice were separately removed at 10, 25, 30,60 and 90 days after birth. Hematoxylin-eosin (H&E) and immunohistochemical staining were performed for HDACs. Gene Expression of HDACs in C57BL/6. NOD-Aec1Aec2 mice salivary glands during the development of Sjögren\'s syndrome-like illness were also analyzed by using the gene expression datasets (GSE 15640).
RESULTS: In the mice salivary gland, HDACs were found to have different localization patterns at various stages of development (10, 25, 30, 60, and 90 days). Apart from HDAC6, ductal cells of salivary glands were the primary sites for HDAC localization. HDAC2, 8, 5, 10 and 11 were expressed at high levels in the salivary gland after birth while HDAC6 showed no expression during postnatal development. This suggests that these HDAC subtypes may have different roles in salivary gland function. In the context of Sjögren\'s syndrome-like illness, HDAC 2, 8 and 10 showed low expression while HDAC1, 6,5,3 and 11 had relatively high expression in the salivary gland.
CONCLUSIONS: This study has provided an important reference for understanding the spatiotemporal-specific expression of HDACs in the salivary gland. These results offer new clues for the experimenters and hold promise for developing innovative therapeutic strategies for salivary gland-related diseases.
METHODS: The major salivary glands of C57/BL6 mice were separately removed at 10, 25, 30,60 and 90 days after birth. Hematoxylin-eosin (H&E) and immunohistochemical staining were performed for HDACs. Gene Expression of HDACs in C57BL/6. NOD-Aec1Aec2 mice salivary glands during the development of Sjögren\'s syndrome-like illness were also analyzed by using the gene expression datasets (GSE 15640).
RESULTS: In the mice salivary gland, HDACs were found to have different localization patterns at various stages of development (10, 25, 30, 60, and 90 days). Apart from HDAC6, ductal cells of salivary glands were the primary sites for HDAC localization. HDAC2, 8, 5, 10 and 11 were expressed at high levels in the salivary gland after birth while HDAC6 showed no expression during postnatal development. This suggests that these HDAC subtypes may have different roles in salivary gland function. In the context of Sjögren\'s syndrome-like illness, HDAC 2, 8 and 10 showed low expression while HDAC1, 6,5,3 and 11 had relatively high expression in the salivary gland.
CONCLUSIONS: This study has provided an important reference for understanding the spatiotemporal-specific expression of HDACs in the salivary gland. These results offer new clues for the experimenters and hold promise for developing innovative therapeutic strategies for salivary gland-related diseases.
摘要：
目的：组蛋白脱乙酰酶（HDAC）是参与基因表达控制的表观遗传调节剂。没有关于HDAC在唾液腺中的表达或亚细胞定位的数据。本研究旨在检查出生后发育过程中唾液腺中HDAC的亚细胞分布。
方法：在出生后10、25、30、60和90天分别摘除C57/BL6小鼠的主要唾液腺。对HDAC进行苏木精-伊红(H&E)和免疫组织化学染色。HDACs在C57BL/6中的基因表达。还通过使用基因表达数据集（GSE15640）分析了在Sjögren综合征样疾病发展过程中的NOD-Aec1Aec2小鼠唾液腺。
结果：在小鼠唾液腺中，发现HDAC在各个发展阶段（10、25、30、60和90天）具有不同的定位模式。除HDAC6外，唾液腺的导管细胞是HDAC定位的主要部位。HDAC2、8、5、10和11在出生后唾液腺中高水平表达，而HDAC6在出生后发育过程中没有表达。这表明这些HDAC亚型在唾液腺功能中可能具有不同的作用。在Sjögren综合征样疾病的背景下，HDAC2、8和10在唾液腺中呈低表达，而HDAC1、6、5、3和11的表达相对较高。
结论：本研究为了解唾液腺中HDAC的时空特异性表达提供了重要参考。这些结果为实验者提供了新的线索，并为开发唾液腺相关疾病的创新治疗策略提供了希望。
方法：在出生后10、25、30、60和90天分别摘除C57/BL6小鼠的主要唾液腺。对HDAC进行苏木精-伊红(H&E)和免疫组织化学染色。HDACs在C57BL/6中的基因表达。还通过使用基因表达数据集（GSE15640）分析了在Sjögren综合征样疾病发展过程中的NOD-Aec1Aec2小鼠唾液腺。
结果：在小鼠唾液腺中，发现HDAC在各个发展阶段（10、25、30、60和90天）具有不同的定位模式。除HDAC6外，唾液腺的导管细胞是HDAC定位的主要部位。HDAC2、8、5、10和11在出生后唾液腺中高水平表达，而HDAC6在出生后发育过程中没有表达。这表明这些HDAC亚型在唾液腺功能中可能具有不同的作用。在Sjögren综合征样疾病的背景下，HDAC2、8和10在唾液腺中呈低表达，而HDAC1、6、5、3和11的表达相对较高。
结论：本研究为了解唾液腺中HDAC的时空特异性表达提供了重要参考。这些结果为实验者提供了新的线索，并为开发唾液腺相关疾病的创新治疗策略提供了希望。
