Abstract：
Aims: Cyanoenone triterpenoids penetrate the CNS, exhibiting biological activity via the nuclear factor E2-related factor (Nrf2) pathway. This is the first report on methods for the quantification of cyanonenone triterpenoids\' distribution in various CNS tissues by LC-MS/MS. Materials & methods: The analyte was extracted from brain tissue homogenate using protein precipitation and supported liquid extraction. Results & conclusion: The assay validated a quantification range of 3.00-3000 ng/g in brain tissue samples as low as 5 mg. All parameters, including interference (≤20% at LLOQ) and accuracy/precision (15%, with 20% at LLOQ), met acceptance criteria. This assay supported a CNS distribution study, analyzing more than 10 mouse brain regions successfully.
摘要：
目的：氰烯酮三萜类化合物渗透中枢神经系统，通过核因子E2相关因子（Nrf2）途径表现出生物活性。这是关于通过LC-MS/MS定量各种中枢神经系统组织中氰基烯酮三萜类化合物分布的方法的第一份报告。材料和方法:使用蛋白沉淀和支持的液体提取从脑组织匀浆中提取分析物。结果和结论:该测定验证了脑组织样品中3.00-3000ng/g的定量范围低至5mg。所有参数,包括干扰(LLOQ≤20%)和准确度/精度(15%，LLOQ为20%)，符合验收标准。该测定支持CNS分布研究，成功分析了10多个小鼠大脑区域。
