PDF(Pubmed)
Abstract：
BACKGROUND: Chagas disease, affecting approximately eight million individuals in tropical regions, is primarily transmitted by vectors. Rhodnius prolixus, a triatomine vector, commonly inhabits in ecotopes with diverse palm tree species, creating optimal conditions for vector proliferation. This study aims to explore the transmission ecology of Trypanosoma cruzi, the causative parasite of Chagas disease, by investigating the feeding patterns and natural infection rates of R. prolixus specimens collected from various wild palm species in the Colombian Orinoco region.
METHODS: To achieve this objective, we sampled 35 individuals from three palm species (Attalea butyracea, Acrocomia aculeata, and Mauritia flexuosa) in a riparian forest in the Casanare department of eastern Colombia, totaling 105 sampled palm trees. DNA was extracted and analyzed from 115 R. prolixus specimens at different developmental stages using quantitative PCR (qPCR) for T. cruzi detection and identification of discrete typing units. Feeding preferences were determined by sequencing the 12S rRNA gene amplicon through next-generation sequencing.
RESULTS: A total of 676 R. prolixus specimens were collected from the sampled palms. The study revealed variation in population densities and developmental stages of R. prolixus among palm tree species, with higher densities observed in A. butyracea and lower densities in M. flexuosa. TcI was the exclusive T. cruzi discrete typing unit (DTU) found, with infection frequency positively correlated with R. prolixus abundance. Insects captured in A. butyracea exhibited higher abundance and infection rates than those from other palm species. The feeding sources comprised 13 mammal species, showing no significant differences between palm species in terms of blood sources. However, Didelphis marsupialis and Homo sapiens were present in all examined R. prolixus, and Dasypus novemcinctus was found in 89.47% of the insects.
CONCLUSIONS: This study highlights the significance of wild palms, particularly A. butyracea, as a substantial risk factor for T. cruzi transmission to humans in these environments. High population densities and infection rates of R. prolixus were observed in each examined palm tree species.
METHODS: To achieve this objective, we sampled 35 individuals from three palm species (Attalea butyracea, Acrocomia aculeata, and Mauritia flexuosa) in a riparian forest in the Casanare department of eastern Colombia, totaling 105 sampled palm trees. DNA was extracted and analyzed from 115 R. prolixus specimens at different developmental stages using quantitative PCR (qPCR) for T. cruzi detection and identification of discrete typing units. Feeding preferences were determined by sequencing the 12S rRNA gene amplicon through next-generation sequencing.
RESULTS: A total of 676 R. prolixus specimens were collected from the sampled palms. The study revealed variation in population densities and developmental stages of R. prolixus among palm tree species, with higher densities observed in A. butyracea and lower densities in M. flexuosa. TcI was the exclusive T. cruzi discrete typing unit (DTU) found, with infection frequency positively correlated with R. prolixus abundance. Insects captured in A. butyracea exhibited higher abundance and infection rates than those from other palm species. The feeding sources comprised 13 mammal species, showing no significant differences between palm species in terms of blood sources. However, Didelphis marsupialis and Homo sapiens were present in all examined R. prolixus, and Dasypus novemcinctus was found in 89.47% of the insects.
CONCLUSIONS: This study highlights the significance of wild palms, particularly A. butyracea, as a substantial risk factor for T. cruzi transmission to humans in these environments. High population densities and infection rates of R. prolixus were observed in each examined palm tree species.
摘要：
背景：查加斯病，影响了热带地区大约800万人，主要通过矢量传输。Rhodniusprolixus,一个triatomine载体,通常栖息在具有不同棕榈树物种的生态圈中，为载体增殖创造最佳条件。本研究旨在探索克氏锥虫的传播生态学,查加斯病的致病寄生虫,通过调查从哥伦比亚奥里诺科地区的各种野生棕榈物种收集的R.prolixus标本的摄食方式和自然感染率。
方法：为了实现这一目标，我们从三个棕榈物种(AttaleaButhyracea，acrocomiaaculeata,和毛里求斯flexuosa）在哥伦比亚东部卡萨纳雷省的河岸森林中，总共105棵棕榈树样本。使用定量PCR（qPCR）从115个不同发育阶段的R.prolixus标本中提取DNA并进行分析，以进行T.cruzi检测和鉴定离散分型单位。通过下一代测序对12SrRNA基因扩增子进行测序来确定喂养偏好。
结果：从采样的手掌中收集了总共676个头孢。该研究揭示了棕榈树物种中R.prolixus的种群密度和发育阶段的变化，在丁香A中观察到较高的密度，而在弯曲分枝杆菌中观察到较低的密度。TcI是发现的独家T.Cruzi离散打字单元（DTU），与感染频率呈正相关。与其他棕榈物种相比，在丁酸A中捕获的昆虫表现出更高的丰度和感染率。摄食来源包括13种哺乳动物，在血液来源方面，棕榈物种之间没有显着差异。然而,所有检查过的R.prolixus中都存在Didelphismarsupialis和智人，在89.47%的昆虫中发现了Dasypusnovemcinctus。
结论：这项研究强调了野生棕榈的重要性，特别是A.丁酸，在这些环境中作为克氏虫传播给人类的重要危险因素。在每个检查的棕榈树物种中观察到高种群密度和高感染率。
方法：为了实现这一目标，我们从三个棕榈物种(AttaleaButhyracea，acrocomiaaculeata,和毛里求斯flexuosa）在哥伦比亚东部卡萨纳雷省的河岸森林中，总共105棵棕榈树样本。使用定量PCR（qPCR）从115个不同发育阶段的R.prolixus标本中提取DNA并进行分析，以进行T.cruzi检测和鉴定离散分型单位。通过下一代测序对12SrRNA基因扩增子进行测序来确定喂养偏好。
结果：从采样的手掌中收集了总共676个头孢。该研究揭示了棕榈树物种中R.prolixus的种群密度和发育阶段的变化，在丁香A中观察到较高的密度，而在弯曲分枝杆菌中观察到较低的密度。TcI是发现的独家T.Cruzi离散打字单元（DTU），与感染频率呈正相关。与其他棕榈物种相比，在丁酸A中捕获的昆虫表现出更高的丰度和感染率。摄食来源包括13种哺乳动物，在血液来源方面，棕榈物种之间没有显着差异。然而,所有检查过的R.prolixus中都存在Didelphismarsupialis和智人，在89.47%的昆虫中发现了Dasypusnovemcinctus。
结论：这项研究强调了野生棕榈的重要性，特别是A.丁酸，在这些环境中作为克氏虫传播给人类的重要危险因素。在每个检查的棕榈树物种中观察到高种群密度和高感染率。
