PDF(Pubmed)
Abstract：
UNASSIGNED: Aspergillosis is a disease that affects several species of birds and causes substantial losses in the poultry business. The purpose of the investigation was to identify the pathogen responsible for a respiratory outbreak among juvenile ducklings.
UNASSIGNED: An epidemic of Aspergillosis infected a total of 800 Muscovy ducks that were being reared in El-Beheira Governorate. Tissue samples were obtained to isolate suspected fungi from diseased birds and the hatchery environment. In addition, identification and molecular characterization were performed on the obtained fungal isolates.
UNASSIGNED: Affected birds displayed acute respiratory manifestations such as difficulty breathing, gasping for air, nasal discharge, and a mortality rate of up to 28.1%. Postmortem examination revealed bronchitis, tracheitis, congested lungs, air sacculitis, severe multifocal granulomatous pneumonia, a congested, enlarged liver, and a congested kidney with nephritis. Mycological examination revealed seven Aspergillus (A.) spp. isolates from ducklings and six from hatcheries. Isolate colonial morphology and microscopical examination were as follows: A. fumigatus, A. niger, Syncephalastrum racemosum, and four untypable isolates. These isolates were further identified by polymerase chain reaction (PCR), and the internal transcribed spacers (ITSs) gene was detected. Four representative isolates were submitted for sequencing and further phylogenetic analysis. The source of duckling infection might be linked to the hatchery environment due to the observed similarity of isolates from both affected birds and the hatchery, as evidenced by phylogenetic analysis.
UNASSIGNED: Our findings demonstrated the significance of appropriate hatchery control in preventing infection in young ducklings. Furthermore, the use of molecular identification techniques would be helpful for tracing the source of infection and rapid diagnosis of Aspergillus in the field.
UNASSIGNED: An epidemic of Aspergillosis infected a total of 800 Muscovy ducks that were being reared in El-Beheira Governorate. Tissue samples were obtained to isolate suspected fungi from diseased birds and the hatchery environment. In addition, identification and molecular characterization were performed on the obtained fungal isolates.
UNASSIGNED: Affected birds displayed acute respiratory manifestations such as difficulty breathing, gasping for air, nasal discharge, and a mortality rate of up to 28.1%. Postmortem examination revealed bronchitis, tracheitis, congested lungs, air sacculitis, severe multifocal granulomatous pneumonia, a congested, enlarged liver, and a congested kidney with nephritis. Mycological examination revealed seven Aspergillus (A.) spp. isolates from ducklings and six from hatcheries. Isolate colonial morphology and microscopical examination were as follows: A. fumigatus, A. niger, Syncephalastrum racemosum, and four untypable isolates. These isolates were further identified by polymerase chain reaction (PCR), and the internal transcribed spacers (ITSs) gene was detected. Four representative isolates were submitted for sequencing and further phylogenetic analysis. The source of duckling infection might be linked to the hatchery environment due to the observed similarity of isolates from both affected birds and the hatchery, as evidenced by phylogenetic analysis.
UNASSIGNED: Our findings demonstrated the significance of appropriate hatchery control in preventing infection in young ducklings. Furthermore, the use of molecular identification techniques would be helpful for tracing the source of infection and rapid diagnosis of Aspergillus in the field.
摘要：
曲霉病是一种影响多种鸟类的疾病，并在家禽业务中造成重大损失。调查的目的是确定引起幼鸭呼吸道暴发的病原体。
■曲霉病的流行感染了在El-Beheira省饲养的800只番鸭。获得组织样品以从患病的鸟类和孵化场环境中分离出可疑的真菌。此外,对获得的真菌分离株进行鉴定和分子表征。
■受影响的鸟类表现出急性呼吸道表现，例如呼吸困难，喘气的空气，鼻腔分泌物,死亡率高达28.1%。尸检显示支气管炎,支气管炎,充血的肺，气囊炎,严重的多灶性肉芽肿肺炎，一个拥挤的，肝脏肿大,肾脏充血伴肾炎.真菌学检查显示有七个曲霉（A.)spp。小鸭和六个孵化场的分离株。隔离菌落形态和显微镜检查如下：烟曲霉，A.尼日尔,汇流排，和四个不可型的分离株。通过聚合酶链反应（PCR）进一步鉴定了这些分离株，并检测到内部转录间隔区（ITS）基因。对四个代表性的分离株进行测序和进一步的系统发育分析。由于观察到来自受影响鸟类和孵化场的分离株的相似性，小鸭感染的来源可能与孵化场环境有关。系统发育分析证明了这一点。
■我们的发现证明了适当的孵化场控制对预防幼鸭感染的重要性。此外，分子鉴定技术的使用将有助于在该领域追踪感染源和快速诊断曲霉。
■曲霉病的流行感染了在El-Beheira省饲养的800只番鸭。获得组织样品以从患病的鸟类和孵化场环境中分离出可疑的真菌。此外,对获得的真菌分离株进行鉴定和分子表征。
■受影响的鸟类表现出急性呼吸道表现，例如呼吸困难，喘气的空气，鼻腔分泌物,死亡率高达28.1%。尸检显示支气管炎,支气管炎,充血的肺，气囊炎,严重的多灶性肉芽肿肺炎，一个拥挤的，肝脏肿大,肾脏充血伴肾炎.真菌学检查显示有七个曲霉（A.)spp。小鸭和六个孵化场的分离株。隔离菌落形态和显微镜检查如下：烟曲霉，A.尼日尔,汇流排，和四个不可型的分离株。通过聚合酶链反应（PCR）进一步鉴定了这些分离株，并检测到内部转录间隔区（ITS）基因。对四个代表性的分离株进行测序和进一步的系统发育分析。由于观察到来自受影响鸟类和孵化场的分离株的相似性，小鸭感染的来源可能与孵化场环境有关。系统发育分析证明了这一点。
■我们的发现证明了适当的孵化场控制对预防幼鸭感染的重要性。此外，分子鉴定技术的使用将有助于在该领域追踪感染源和快速诊断曲霉。
