PDF(Pubmed)
Abstract：
Failure to adequately reconstruct the tendon-to-bone interface constitutes the primary etiology underlying rotator cuff retear after surgery. The purpose of this study is to construct a dynamic chondroitin sulfate and chitosan hydrogel scaffold (CHS) with bone morphogenetic protein 2 (BMP2), then seed tendon stem cells (TSCs) on BMP2-CHS for the rotator cuff reconstruction of tendon-to-bone interface. In this dynamic hydrogel system, the scaffold could not only have good biocompatibility and degradability but also significantly promote the proliferation and differentiation of TSCs. The ability of BMP2-CHS combined with TSCs to promote regeneration of tendon-to-bone interface was further verified in the rabbit rotator cuff tear model. The results showed that BMP2-CHS combined with TSCs could induce considerable collagen, fibrocartilage, and bone arrangement and growth at the tendon-to-bone interface and promote the biomechanical properties. Overall, TSCs seeded on CHS with BMP2 can enhance tendon-to-bone healing and provide a new possibility for improving the poor prognosis of rotator cuff surgery.
摘要：
未能充分重建肌腱-骨骼界面是手术后肩袖再撕裂的主要病因。本研究的目的是构建具有骨形态发生蛋白2（BMP2）的动态硫酸软骨素和壳聚糖水凝胶支架（CHS），然后在BMP2-CHS上种子肌腱干细胞（TSCs）用于肌腱-骨界面的肩袖重建。在这个动态水凝胶系统中，该支架不仅具有良好的生物相容性和可降解性,而且能显著促进TSCs的增殖和分化。在兔肩袖撕裂模型中进一步验证了BMP2-CHS联合TSCs促进肌腱-骨界面再生的能力。结果表明,BMP2-CHS与TSCs结合可诱导大量胶原,纤维软骨,以及肌腱-骨界面处的骨排列和生长，并促进生物力学特性。总的来说,在CHS上接种BMP2的TSCs可以增强肌腱-骨的愈合，为改善肩袖手术的不良预后提供了新的可能。
