PDF(Pubmed)
Abstract：
A study was conducted to investigate the population genetic structure and breeding pattern of 140 tropical bed bugs, Cimex hemipterus (F.) (Hemiptera: Cimicidae), collected from 14 infested sites in major cities in Iraq. The samples were genotyped using a set of 7 polymorphic microsatellite markers. High genetic variety was seen among populations, with an average of 2-9 alleles per locus. The number of alleles across 7 microsatellite loci was between 6 and 18. There was a notable disparity in the alleles per loci when comparing the overall population to those within it. The overall population exhibited an average observed heterozygosity of 0.175 and an average expected heterozygosity of 0.730. Among the population, the average observed heterozygosity was 0.173, while the average expected heterozygosity was 0.673. Analysis of molecular variance (AMOVA) revealed that 93% of the genetic variability was within the populations, and 7% was among them. The genetic differentiation coefficient (FST = 0.045), indicates a low degree of genetic differentiation and a high degree of inbreeding (FIS = 0.761), as indicated by notably significant positive inbreeding coefficients. Admixed individuals were revealed using STRUCTURE and neighbor-joining phylogenetic trees, demonstrating moderate gene flow between populations and a lack of genetic structure in the regional groups. Thus, both active dispersion and human-mediated dispersion possess the potential to influence the low population genetic structure of tropical bed bug C. hemipterus populations in Iraq, which can have implications toward tropical bed bug and management strategies.
摘要：
研究了140只热带臭虫的种群遗传结构和繁殖模式,Cimex半翅目(F.)(半翅目：Cimicidae)，从伊拉克主要城市的14个出没地点收集。使用一组7个多态微卫星标记对样品进行基因分型。在种群中发现了高遗传变异，每个基因座平均有2-9个等位基因。跨越7个微卫星位点的等位基因数目在6到18之间。当将总体人群与其中的人群进行比较时，每个基因座的等位基因存在显着差异。总体群体表现出0.175的平均观察杂合性和0.730的平均预期杂合性。在人口中,平均观察杂合度为0.173,平均期望杂合度为0.673。分子方差分析（AMOVA）显示，93％的遗传变异在种群内，其中7%。遗传分化系数(FST=0.045),表明遗传分化程度低，近交程度高（FIS=0.761），如显着的正近交系数所示。使用结构和邻居连接系统发育树揭示混合个体，表明种群之间的基因流动适度，区域群体缺乏遗传结构。因此，主动分散和人类介导的分散都有可能影响伊拉克热带臭虫C.半翅目种群的低种群遗传结构，这可能会对热带臭虫和管理策略产生影响。
