Abstract：
The harlequin sandsmelt (Parapercis pulchella) is a female-to-male sex change fish in which functional females possess ovotestes that consist of both ovarian and testicular tissues. These features indicate that this species could be an excellent model for studying the flexibility of sex differentiation in vertebrates. However, genetic resources in this species have so far been limited. Therefore, in this study, the reference transcriptome of this fish was constructed through RNA-sequencing, de novo transcriptome assembly, superTranscripts construction, and functional annotations. To obtain as many genes as possible, RNA was extracted from various tissues (brains, gills, hearts, livers, guts, and gonads) and various sexual stages (females, individuals during sex change, and males) and then subjected to sequencing and downstream analyses. As a result, 91,884 representative transcripts with 32,627 protein-coding sequences were generated. 72.2% of protein-coding sequences (23,566 sequences) were functionally annotated. Also, our analysis shows that the superTranscripts method effectively removes redundant sequences from raw-assembled data compared with other strategies. The resultant dataset is a valuable resource for future molecular developmental studies on sex change in P. pulchella.
摘要：
丑角solsmelt（Parapercispulchella）是一种雌性到雄性的变性鱼，其中功能性雌性拥有由卵巢和睾丸组织组成的卵卵。这些特征表明，该物种可能是研究脊椎动物性别分化灵活性的绝佳模型。然而,到目前为止，该物种的遗传资源有限。因此,在这项研究中，这种鱼的参考转录组是通过RNA测序构建的，从头转录组组装，superTranscripts构建，和功能注释。为了获得尽可能多的基因，从各种组织中提取RNA(大脑，ill,心,肝脏,胆量,和性腺)和各种性阶段(女性，个体在性别变化期间，和雄性），然后进行测序和下游分析。因此,产生具有32,627个蛋白质编码序列的91,884个代表性转录物。72.2%的蛋白质编码序列(23,566条序列)被功能注释。此外，我们的分析表明，与其他策略相比，superTranscripts方法有效地从原始组装数据中删除了冗余序列。所得的数据集是未来有关P.pulchella性别变化的分子发育研究的宝贵资源。
