Abstract：
Scaffold design is one of the three most essential parts of tissue engineering. Platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) have been used in clinics and regenerative medicine for years. However, the temporal release of their growth factors limits their efficacy in tissue engineering. In the present study, we planned to synthesize nanofibrous scaffolds with the incorporation of PRP and PRF by electrospinning method to evaluate the effect of the release of PRP and PRF growth factors on osteogenic gene expression, calcification, proliferation, and cell adhesion of human bone marrow mesenchymal stem cell (h-BMSC) as they are part of scaffold structures. Therefore, we combined PRP/PRF, derived from the centrifugation of whole blood, with gelatin and Polycaprolactone (PCL) and produced nanofibrous electrospun PCL/Gel/PRP and PCL/Gel/PRF scaffolds. Three groups of scaffolds were fabricated, and h-BMSCs were seeded on them: (1) PCL/Gel; (2) PCL/Gel/PRP; (3) PCL/Gel/PRF. MTS assay was performed to assess cell proliferation and adhesion, and alizarin red staining confirmed the formation of bone minerals during the experiment. The result indicated that PCL/Gel did not have any better outcomes than the PRP and PRF group in any study variants after the first day of the experiment. PCL/gelatin/PRF was more successful regarding cell proliferation and adhesion. Although PCL/gelatin/PRP showed more promising results on the last day of the experiment in mineralization and osteogenic gene expression, except RUNX2, in which the difference with PCL/gelatin/PRF group was not significant.
摘要：
支架设计是组织工程的三个最重要的部分之一。富血小板血浆（PRP）和富血小板纤维蛋白（PRF）已在临床和再生医学中使用多年。然而,其生长因子的瞬时释放限制了其在组织工程中的功效。在本研究中,我们计划通过静电纺丝法合成掺入PRP和PRF的纳米纤维支架，以评估PRP和PRF生长因子的释放对成骨基因表达的影响，钙化,扩散,和人骨髓间充质干细胞（h-BMSC）的细胞粘附，因为它们是支架结构的一部分。因此,我们结合了PRP/PRF，来自全血的离心，与明胶和聚己内酯（PCL）和生产的纳米纤维电纺PCL/凝胶/PRP和PCL/凝胶/PRF支架。制造了三组支架，和h-BMSCs接种在它们上:(1)PCL/Gel;(2)PCL/Gel/PRP;(3)PCL/Gel/PRF。进行MTS测定以评估细胞增殖和粘附，茜素红染色证实了实验过程中骨矿物质的形成。结果表明,在实验的第一天之后,在任何研究变体中,PCL/Gel没有比PRP和PRF组有任何更好的结果。PCL/明胶/PRF在细胞增殖和粘附方面更成功。尽管PCL/明胶/PRP在矿化和成骨基因表达实验的最后一天显示出更有希望的结果，除RUNX2外，其与PCL/明胶/PRF组差异无统计学意义。
