Abstract：
The present case series aims to investigate the use of polynucleotides mixed with hyaluronic acid (PNs-HA) in the form of gel to promote bone regeneration in horizontal alveolar defects. Overall, 6 adult patients underwent localized horizontal guided bone regeneration by means of xenogeneic bone substitute and a resorbable barrier with a staged approach. The graft consisted in a mixture of deproteinized bovine bone mineral (DBBM) particles and PNs-HA gel in a 3:1 ratio, respectively. The material was covered by a resorbable collagen membrane fixed with pins to the underlying bone. Implant placement was performed after 5 months. Healing proceeded uneventfully, and, upon re-entry, the graft appeared well vascularized and firmly attached to the recipient bone. Histologically, the regenerated bone appeared highly mineralized, well-organized in lamellae and totally embedding the residual granules of the biomaterial. Histomorphometric evaluations revealed that newly formed bone occupied on average 41.2% ± 12.4% of the analysed samples. Linear measurements performed on CBCT scans yielded an overall linear horizontal bone gain of 4.91 ± 0.88 mm. These data suggest that a mixture of DBBM and PNs-HA can be safely used to promote bone regeneration in case of horizontal alveolar defects.
摘要：
本病例系列旨在研究使用与凝胶形式的透明质酸（PNs-HA）混合的多核苷酸来促进水平牙槽缺损中的骨再生。总的来说,6例成人患者通过异种骨替代物和可吸收屏障分阶段进行局部水平引导骨再生。移植物由3：1比例的脱蛋白牛骨矿物质（DBBM）颗粒和PNs-HA凝胶的混合物组成，分别。该材料由可再吸收的胶原膜覆盖,所述胶原膜用销钉固定到下面的骨上。5个月后进行植入物放置。治疗进展顺利，and,在重新进入时，移植物血管化良好，并牢固地附着在受体骨上。组织学上,再生骨出现高度矿化，在薄片中组织良好，并完全嵌入生物材料的残留颗粒。组织形态计量学评估显示，新形成的骨平均占分析样品的41.2％±12.4％。在CBCT扫描上进行的线性测量产生4.91±0.88mm的整体线性水平骨增益。这些数据表明，在水平牙槽缺损的情况下，DBBM和PNs-HA的混合物可以安全地用于促进骨再生。
