PDF(Pubmed)
Abstract：
Two adult male Puerto Rican crested anoles (Anolis cristatellus cristatellus) housed in a research facility were presented with debilitation and were euthanized. On autopsy, anole 1 had a large cystic white structure in the left pelvic limb, which protruded through the ruptured epidermis, and a large, poorly demarcated swelling in the right caudal abdomen. Anole 2 had masses in the mid-dorsum, caudal dorsum, left pelvic limb, and tail. These masses contained variably sized cestode larvae, which ruptured into the coelomic cavity. Evaluation of the larvae revealed a thickened and wrinkled anterior end, with a cleft-like invagination, consistent with either a plerocercoid sparganum or a tetrathyridium. Histologically, several cestode larvae were contained in the body wall of both anoles. These were up to 650 μm in diameter, with a thin tegument and a spongy parenchyma. The spongy parenchyma contained numerous, up to 30 μm diameter, sharply demarcated, basophilic-to-black structures (calcareous corpuscles). There was pneumonia and hepatitis in anole 2, suggestive of potential secondary infection subsequent to immunosuppression. Molecular amplification of the cytochrome C oxidase subunit 1 revealed 100% homology for the COX1 gene of the diphyllobothriid tapeworm Spirometra erinaceieuropaei, also known as Spirometra mansoni.
摘要：
安置在研究设施中的两名成年男性波多黎各凤头anoles（Anoliscristatelluscristatellus）被认为衰弱并被安乐死。尸检时,Anole1在左骨盆肢体有一个大的囊性白色结构，通过破裂的表皮突出，和一个大的，右侧尾腹部肿胀界限不清。Anole2在背部中部有肿块，尾部背部,左骨盆肢,和尾巴。这些肿块包含大小不同的昆虫幼虫，破裂进入体腔。对幼虫的评估显示前端增厚且起皱，有一个像裂缝一样的内陷，与角砾岩或四虫一致。组织学上,两个anoles的体壁中都包含了几个c虫幼虫。这些直径高达650μm，薄外皮和海绵状薄壁组织。海绵状薄壁组织包含许多，直径可达30μm，严格划界，嗜碱性至黑色结构（钙质小体）。Anole2中有肺炎和肝炎，提示免疫抑制后可能继发感染。细胞色素C氧化酶亚基1的分子扩增显示，二叶虫的COX1基因具有100％的同源性，也被称为Spirometramansoni。
