PDF(Pubmed)
Abstract：
A One Health approach has been key to monitoring the COVID-19 pandemic, as human and veterinary medical professionals jointly met the demands for an extraordinary testing effort for SARS-CoV-2. Veterinary diagnostic laboratories continue to monitor SARS-CoV-2 infection in animals, furthering the understanding of zoonotic transmission dynamics between humans and animals. A RT-PCR assay is a primary animal screening tool established within validation and verification guidelines provided by the American Association of Veterinary Laboratory Diagnosticians (AAVLD), World Organisation for Animal Health (WOAH), and the U.S. Food and Drug Administration (FDA). However, differences in sample matrices, RNA extraction methods, instrument platforms, gene targets, and cutoff values may affect test outcomes. Therefore, targeted validation for a new sample matrix used in any PCR assay is critical. We evaluated a COVID-19 assay for the detection of SARS-CoV-2 in feline and canine lung homogenates and oral swab samples. We used the commercial Applied Biosystems MagMAX Viral/Pathogen II (MVP II) nucleic acid isolation kit and TaqPath COVID-19 Combo kit, which are validated for a variety of human samples, including nasopharyngeal and oropharyngeal swab samples. Our masked test showed a high detection rate and no false-positive or false-negative results, supporting sample extension to include feline oral swab samples. Our study is a prime example of One Health, illustrating how a COVID-19 assay designed for human testing can be adapted and used to detect SARS-CoV-2 in oral swab samples from cats and likely dogs, but not lung homogenates.
摘要：
一个健康的方法一直是监测COVID-19大流行的关键，因为人类和兽医医疗专业人员共同满足了SARS-CoV-2非凡测试工作的需求。兽医诊断实验室继续监测动物身上的SARS-CoV-2感染,进一步了解人与动物之间的人畜共患传播动力学。RT-PCR测定法是在美国兽医实验室诊断医师协会（AAVLD）提供的验证和验证指南中建立的主要动物筛选工具。世界动物卫生组织（WOAH）和美国食品和药物管理局（FDA）。然而,样本矩阵的差异，RNA提取方法,仪器平台,基因靶标，和截止值可能会影响测试结果。因此,任何PCR检测中使用的新样品基质的靶向验证都是至关重要的.我们评估了在猫和犬肺匀浆和口腔拭子样品中检测SARS-CoV-2的COVID-19测定法。我们使用了商业应用生物系统MagMAX病毒/病原体II（MVPII）核酸分离试剂盒和TaqPathCOVID-19组合试剂盒，对各种人类样本进行了验证，包括鼻咽和口咽拭子样本。我们的蒙面检测显示检出率高，没有假阳性或假阴性结果，支持样本延伸，包括猫科动物口腔拭子样本。我们的研究是一个健康的典型例子，说明了设计用于人体测试的COVID-19测定法如何适用于检测猫和可能的狗的口腔拭子样本中的SARS-CoV-2，但不是肺匀浆.
