PDF(Pubmed)
Abstract：
Vibrio parahaemolyticus is a significant cause of foodborne illness, and its incidence worldwide is on the rise. It is thus imperative to develop a straightforward and efficient method for typing strains of this pathogen. In this study, we conducted a pangenome analysis of 75 complete genomes of V. parahaemolyticus and identified the core gene mtlA with the highest degree of variation, which distinguished 44 strains and outperformed traditional seven-gene-based MLST when combined with aer, another core gene with high degree of variation. The mtlA gene had higher resolution to type strains with a close relationship compared to the traditional MLST genes in the phylogenetic tree built by core genomes. Strong positive selection was also detected in the gene mtlA (ω > 1), representing adaptive and evolution in response to the environment. Therefore, the panel of gene mtlA and aer may serve as a tool for the typing of V. parahaemolyticus, potentially contributing to the prevention and control of this foodborne disease.
摘要：
副溶血性弧菌是食源性疾病的重要原因，它在世界范围内的发病率正在上升。因此，必须开发一种直接有效的方法来对该病原体的菌株进行分型。在这项研究中,我们对75个完整的副溶血性弧菌基因组进行了pangenome分析，并确定了变异程度最高的核心基因mtlA，它区分了44个菌株，在与aer结合时优于传统的基于七基因的MLST，另一个高度变异的核心基因。在核心基因组构建的系统发育树中，与传统的MLST基因相比，mtlA基因对具有密切关系的类型菌株具有更高的分辨率。在基因mtlA(ω>1)中也检测到强阳性选择，代表对环境的适应性和进化。因此,mtlA和aer基因组可以作为副溶血性弧菌分型的工具，可能有助于预防和控制这种食源性疾病。
