PDF(Pubmed)
Abstract：
The Kazakh horse, renowned for its excellence as a breed, exhibits distinctive reproductive traits characterized by early maturity and seasonal estrus. While normal reproductive function is crucial for ensuring the breeding and expansion of the Kazakh horse population, a noteworthy decline in reproductive capabilities is observed after reaching 14 years of age.
In this study, ovarian granulosa cells (GCs) were meticulously collected from Kazakh horses aged 1, 2, 7, and above 15 years old (excluding 15 years old) for whole transcriptome sequencing.
The analysis identified and selected differentially expressed mRNAs, lncRNAs, miRNAs, and circRNAs for each age group, followed by a thorough examination through GO enrichment analysis. The study uncovered significant variations in the expression profiles of mRNAs, lncRNAs, miRNAs, and circRNAs within GCs at different stages of maturity. Notably, eca-miR-486-3p and miR-486-y exhibited the highest degree of connectivity. Subsequent GO, KEGG, PPI, and ceRNA network analyses elucidated that the differentially expressed target genes actively participate in signaling pathways associated with cell proliferation, apoptosis, and hormonal regulation. These pathways include but are not limited to the MAPK signaling pathway, Hippo signaling pathway, Wnt signaling pathway, Calcium signaling pathway, Aldosterone synthesis and secretion, Cellular senescence, and NF-kappa B signaling pathway-essentially encompassing signal transduction pathways crucial to reproductive processes.
This research significantly contributes to unraveling the molecular mechanisms governing follicular development in Kazakh horses. It establishes and preliminarily validates a differential regulatory network involving lncRNA-miRNA-mRNA, intricately associated with processes such as cell proliferation, differentiation, and apoptosis and integral to the developmental intricacies of stromal follicles. The findings of this study provide a solid theoretical foundation for delving deeper into the realm of reproductive aging in Kazakh mares, presenting itself as a pivotal regulatory pathway in the context of horse ovarian development.
In this study, ovarian granulosa cells (GCs) were meticulously collected from Kazakh horses aged 1, 2, 7, and above 15 years old (excluding 15 years old) for whole transcriptome sequencing.
The analysis identified and selected differentially expressed mRNAs, lncRNAs, miRNAs, and circRNAs for each age group, followed by a thorough examination through GO enrichment analysis. The study uncovered significant variations in the expression profiles of mRNAs, lncRNAs, miRNAs, and circRNAs within GCs at different stages of maturity. Notably, eca-miR-486-3p and miR-486-y exhibited the highest degree of connectivity. Subsequent GO, KEGG, PPI, and ceRNA network analyses elucidated that the differentially expressed target genes actively participate in signaling pathways associated with cell proliferation, apoptosis, and hormonal regulation. These pathways include but are not limited to the MAPK signaling pathway, Hippo signaling pathway, Wnt signaling pathway, Calcium signaling pathway, Aldosterone synthesis and secretion, Cellular senescence, and NF-kappa B signaling pathway-essentially encompassing signal transduction pathways crucial to reproductive processes.
This research significantly contributes to unraveling the molecular mechanisms governing follicular development in Kazakh horses. It establishes and preliminarily validates a differential regulatory network involving lncRNA-miRNA-mRNA, intricately associated with processes such as cell proliferation, differentiation, and apoptosis and integral to the developmental intricacies of stromal follicles. The findings of this study provide a solid theoretical foundation for delving deeper into the realm of reproductive aging in Kazakh mares, presenting itself as a pivotal regulatory pathway in the context of horse ovarian development.
摘要：
■哈萨克马,以其卓越的品种而闻名，表现出独特的生殖特征，其特征是早熟和季节性发情。虽然正常的生殖功能对于确保哈萨克马种群的繁殖和扩大至关重要，达到14岁后，生殖能力显着下降。
■在这项研究中，从年龄1,2,7和15岁以上(不包括15岁)的哈萨克族马精心收集卵巢颗粒细胞(GC),用于全转录组测序.
■分析鉴定并选择了差异表达的mRNA，lncRNAs,miRNA,和每个年龄组的circRNAs，然后通过GO富集分析进行彻底检查。该研究揭示了mRNA表达谱的显著差异，lncRNAs,miRNA,和不同成熟阶段GC内的circRNAs。值得注意的是,eca-miR-486-3p和miR-486-y显示最高程度的连接。后续GO,KEGG,PPI,和ceRNA网络分析阐明，差异表达的靶基因积极参与与细胞增殖相关的信号通路，凋亡，和荷尔蒙调节。这些途径包括但不限于MAPK信号通路,河马信号通路,Wnt信号通路,钙信号通路,醛固酮的合成和分泌，细胞衰老,和NF-κB信号通路-基本上包括对生殖过程至关重要的信号转导通路。
■这项研究极大地有助于揭示哈萨克族马卵泡发育的分子机制。它建立并初步验证了涉及lncRNA-miRNA-mRNA的差异调控网络，与细胞增殖等过程密切相关，分化,和细胞凋亡以及基质卵泡发育复杂性的组成部分。本研究的发现为深入研究哈萨克母马的生殖衰老领域提供了坚实的理论基础。在马卵巢发育的背景下，它是一条关键的调节途径。
■在这项研究中，从年龄1,2,7和15岁以上(不包括15岁)的哈萨克族马精心收集卵巢颗粒细胞(GC),用于全转录组测序.
■分析鉴定并选择了差异表达的mRNA，lncRNAs,miRNA,和每个年龄组的circRNAs，然后通过GO富集分析进行彻底检查。该研究揭示了mRNA表达谱的显著差异，lncRNAs,miRNA,和不同成熟阶段GC内的circRNAs。值得注意的是,eca-miR-486-3p和miR-486-y显示最高程度的连接。后续GO,KEGG,PPI,和ceRNA网络分析阐明，差异表达的靶基因积极参与与细胞增殖相关的信号通路，凋亡，和荷尔蒙调节。这些途径包括但不限于MAPK信号通路,河马信号通路,Wnt信号通路,钙信号通路,醛固酮的合成和分泌，细胞衰老,和NF-κB信号通路-基本上包括对生殖过程至关重要的信号转导通路。
■这项研究极大地有助于揭示哈萨克族马卵泡发育的分子机制。它建立并初步验证了涉及lncRNA-miRNA-mRNA的差异调控网络，与细胞增殖等过程密切相关，分化,和细胞凋亡以及基质卵泡发育复杂性的组成部分。本研究的发现为深入研究哈萨克母马的生殖衰老领域提供了坚实的理论基础。在马卵巢发育的背景下，它是一条关键的调节途径。
