PDF(Pubmed)
Abstract：
BACKGROUND: The diagnosis and genetic characterization of Toxoplasma gondii (T. gondii) infection can make a significant influence to the prevention of the dangerous consequences of toxoplasmosis, particularly in immunocompromised people.
OBJECTIVE: The aim of this investigation was to assess the frequency and genotyping of T. gondii in blood samples of patients with hemodialysis.
METHODS: In the current investigation, a total of 379 blood samples were taken from subjects with hemodialysis who were referred to teaching hospital of Ahvaz in the southwest of Iran. The samples were evaluated using the Nested PCR by targeting the B1 gene, and then, sequencing and phylogenetic tree were constructed.
RESULTS: T. gondii DNA was found in 112 (29.55%) of the blood samples by Nested PCR. Amplicons from T. gondii revealed high identity with GenBank sequences. The phylogenetic analysis revealed that all sequences were closely related to Type I of T. gondii.
CONCLUSIONS: Because of the high incidence of toxoplasmosis with type I prevalent in hemodialysis patients, we recommend a systematic screening for toxoplasmosis to carry out for monitoring the possible dissemination of toxoplasmosis during hemodialysis.
OBJECTIVE: The aim of this investigation was to assess the frequency and genotyping of T. gondii in blood samples of patients with hemodialysis.
METHODS: In the current investigation, a total of 379 blood samples were taken from subjects with hemodialysis who were referred to teaching hospital of Ahvaz in the southwest of Iran. The samples were evaluated using the Nested PCR by targeting the B1 gene, and then, sequencing and phylogenetic tree were constructed.
RESULTS: T. gondii DNA was found in 112 (29.55%) of the blood samples by Nested PCR. Amplicons from T. gondii revealed high identity with GenBank sequences. The phylogenetic analysis revealed that all sequences were closely related to Type I of T. gondii.
CONCLUSIONS: Because of the high incidence of toxoplasmosis with type I prevalent in hemodialysis patients, we recommend a systematic screening for toxoplasmosis to carry out for monitoring the possible dissemination of toxoplasmosis during hemodialysis.
摘要：
背景：弓形虫的诊断和遗传特征（T。gondii）感染可以对预防弓形虫病的危险后果产生重大影响，特别是在免疫功能低下的人群中。
目的：本研究的目的是评估血液透析患者血液样本中弓形虫的频率和基因分型。
方法：在当前的调查中，共有379份血液样本取自血液透析患者,他们被转诊到伊朗西南部的阿瓦士教学医院.使用巢式PCR通过靶向B1基因来评估样品，然后,测序和系统发育树的构建。
结果：T.巢式PCR在112份（29.55％）的血液样本中发现了刚地DNA。来自弓形虫的扩增子显示出与GenBank序列的高度同一性。系统发育分析表明，所有序列均与弓形虫I型密切相关。
结论：由于血液透析患者中I型弓形虫病的发病率高，我们建议对弓形虫病进行系统筛查，以监测血液透析过程中弓形虫病的可能传播。
目的：本研究的目的是评估血液透析患者血液样本中弓形虫的频率和基因分型。
方法：在当前的调查中，共有379份血液样本取自血液透析患者,他们被转诊到伊朗西南部的阿瓦士教学医院.使用巢式PCR通过靶向B1基因来评估样品，然后,测序和系统发育树的构建。
结果：T.巢式PCR在112份（29.55％）的血液样本中发现了刚地DNA。来自弓形虫的扩增子显示出与GenBank序列的高度同一性。系统发育分析表明，所有序列均与弓形虫I型密切相关。
结论：由于血液透析患者中I型弓形虫病的发病率高，我们建议对弓形虫病进行系统筛查，以监测血液透析过程中弓形虫病的可能传播。
