Abstract：
Biosensors have emerged as ideal analytical devices for various bio-applications owing to their low cost, convenience, and portability, which offer great potential for improving global healthcare. DNA self-assembly techniques have been enriched with the development of innovative amplification strategies, such as dispersion-to-localization of catalytic hairpin assembly, and dumbbell hybridization chain reaction, which hold great significance for building biosensors capable of realizing sensitive, rapid and multiplexed detection of pathogenic microorganisms. Here, focusing primarily on the signal amplification strategies based on DNA self-assembly, we concisely summarized the strengths and weaknesses of diverse isothermal nucleic acid amplification techniques. Subsequently, both single-layer and cascade amplification strategies based on traditional catalytic hairpin assembly and hybridization chain reaction were critically explored. Furthermore, a comprehensive overview of the recent advances in DNA self-assembled biosensors for the detection of pathogenic microorganisms is presented to summarize methods for biorecognition and signal amplification. Finally, a brief discussion is provided about the current challenges and future directions of DNA self-assembled biosensors.
摘要：
由于其低成本，生物传感器已成为各种生物应用的理想分析设备。便利性，和便携性，这为改善全球医疗保健提供了巨大的潜力。随着创新扩增策略的发展，DNA自组装技术得到了丰富，如分散到局部的催化发夹组件，和哑铃杂交链式反应，这对构建能够实现灵敏的生物传感器具有重要意义，快速和多重检测病原微生物。这里,主要集中在基于DNA自组装的信号放大策略，我们简要总结了各种等温核酸扩增技术的优缺点。随后，对基于传统催化发夹组装和杂交链反应的单层和级联扩增策略进行了严格的探索.此外，综述了近年来用于检测病原微生物的DNA自组装生物传感器的研究进展，总结了生物识别和信号放大的方法。最后,简要讨论了DNA自组装生物传感器的当前挑战和未来发展方向。
