Abstract：
BACKGROUND: We investigated the impact of using canola meal (CM) or corn distillers dried grain soluble (cDDGS) in place of soybean meal (SBM) in low-crude-protein diets supplemented with amino acids (AA) on AA digestibility, gut morphometrics, and AA transporter genes in broiler chicken. On day 0, 540 Cobb 500 male broilers were allocated to six diets in 36-floor pens. The positive control (PC) was a corn-SBM diet with adequate crude protein (CP). The CP level of negative control (NC) was decreased by 45 and 40 g kg-1 relative to PC for grower and finisher phases, respectively. The subsequent two diets had the same CP levels as NC but with cDDGS added at 50 or 125 g kg-1. The last two diets had the same CP as NC but with CM added at 50 or 100 g kg-1.
RESULTS: Dietary CP reduction in corn-SBM diets increased (P < 0.05) the digestibility of Lys (88.5%), Met (90.7%), Thr (77.4%), Cys (80.7%), and Gly (84.7%). Increasing levels of cDDGS linearly decreased (P < 0.05) the digestibility of Asp, Cys, Glu, and Ser, whereas increasing CM level linearly decreased (P < 0.05) the digestibility of Cys, Pro, and Ser. The CP reduction in corn-SBM diets produced downward expression of peptide transporter1 and decreased (P < 0.05) absolute pancreas and ileum weight and length of jejunum and ileum.
CONCLUSIONS: Partial replacement of SBM with alternative protein feedstuffs (cDDGS or CM) in low-CP diets had minimal effects on AA digestibility and mRNA levels of peptides and AA transporters. © 2024 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
RESULTS: Dietary CP reduction in corn-SBM diets increased (P < 0.05) the digestibility of Lys (88.5%), Met (90.7%), Thr (77.4%), Cys (80.7%), and Gly (84.7%). Increasing levels of cDDGS linearly decreased (P < 0.05) the digestibility of Asp, Cys, Glu, and Ser, whereas increasing CM level linearly decreased (P < 0.05) the digestibility of Cys, Pro, and Ser. The CP reduction in corn-SBM diets produced downward expression of peptide transporter1 and decreased (P < 0.05) absolute pancreas and ileum weight and length of jejunum and ileum.
CONCLUSIONS: Partial replacement of SBM with alternative protein feedstuffs (cDDGS or CM) in low-CP diets had minimal effects on AA digestibility and mRNA levels of peptides and AA transporters. © 2024 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
摘要：
背景：我们研究了在补充氨基酸（AA）的低粗蛋白饮食中使用油菜粉（CM）或玉米干谷物可溶物（cDDGS）代替大豆粉（SBM）对AA消化率的影响，肠道形态计量学，和AA转运蛋白基因在肉鸡中的应用。在第0天，将540Cobb500雄性肉鸡分配给36地板围栏中的六种饮食。阳性对照(PC)是具有足够粗蛋白(CP)的玉米-SBM饮食。阴性对照（NC）的CP水平相对于生长期和后整理期的PC降低了45和40gkg-1，分别。随后的两种饮食具有与NC相同的CP水平,但是以50或125gkg-1添加cDDGS。最后两种饮食的CP与NC相同，但CM的添加量为50或100gkg-1。
结果：玉米-SBM日粮中CP的减少增加（P<0.05）Lys的消化率（88.5％），Met（90.7%），Thr（77.4%），Cys（80.7%），和Gly（84.7%）。增加cDDGS的水平线性降低（P<0.05）Asp的消化率，Cys,Glu,Ser,而增加CM水平线性降低（P<0.05）Cys的消化率，Pro,还有Ser.玉米-SBM日粮中CP的减少导致肽转运蛋白1的表达下降，并降低（P<0.05）胰腺和回肠的绝对重量以及空肠和回肠的长度。
结论：在低CP饮食中，用替代蛋白质饲料（cDDGS或CM）部分替代SBM对AA消化率以及肽和AA转运蛋白的mRNA水平影响最小。©2024作者约翰·威利父子有限公司代表化学工业学会出版的《食品和农业科学杂志》。
结果：玉米-SBM日粮中CP的减少增加（P<0.05）Lys的消化率（88.5％），Met（90.7%），Thr（77.4%），Cys（80.7%），和Gly（84.7%）。增加cDDGS的水平线性降低（P<0.05）Asp的消化率，Cys,Glu,Ser,而增加CM水平线性降低（P<0.05）Cys的消化率，Pro,还有Ser.玉米-SBM日粮中CP的减少导致肽转运蛋白1的表达下降，并降低（P<0.05）胰腺和回肠的绝对重量以及空肠和回肠的长度。
结论：在低CP饮食中，用替代蛋白质饲料（cDDGS或CM）部分替代SBM对AA消化率以及肽和AA转运蛋白的mRNA水平影响最小。©2024作者约翰·威利父子有限公司代表化学工业学会出版的《食品和农业科学杂志》。
