PDF(Pubmed)
Abstract：
BACKGROUND: Erythropoietin-producing hepatocellular (EPH) receptors are the largest known family of receptor tyrosine kinases characterized in humans. These proteins are involved in tissue organization, synaptic plasticity, vascular development and the progression of various diseases including cancer. The Erythropoietin-producing hepatocellular receptor tyrosine kinase member EphB6 is a pseudokinase which has not attracted an equivalent amount of interest as its enzymatically-active counterparts. The aim of this study was to assess the expression of EphB6 in pituitary tumors.
RESULTS: Human normal pituitaries and pituitary tumors were examined for EphB6 mRNA expression using real-time PCR and for EphB6 protein by immunohistochemistry and Western blotting. EphB6 was highly expressed in non-functioning pituitary neuroendocrine tumors (NF-PitNETs) versus the normal pituitary and GH-secreting PitNETs. EphB6 mRNA expression was correlated with tumor size.
CONCLUSIONS: Our results suggest EphB6 aberrant expression in NF-PitNETs. Future studies are warranted to determine the role and significance of EphB6 in NF-PitNETs tumorigenesis.
RESULTS: Human normal pituitaries and pituitary tumors were examined for EphB6 mRNA expression using real-time PCR and for EphB6 protein by immunohistochemistry and Western blotting. EphB6 was highly expressed in non-functioning pituitary neuroendocrine tumors (NF-PitNETs) versus the normal pituitary and GH-secreting PitNETs. EphB6 mRNA expression was correlated with tumor size.
CONCLUSIONS: Our results suggest EphB6 aberrant expression in NF-PitNETs. Future studies are warranted to determine the role and significance of EphB6 in NF-PitNETs tumorigenesis.
摘要：
背景：产生促红细胞生成素的肝细胞（EPH）受体是人类中最大的已知受体酪氨酸激酶家族。这些蛋白质参与组织组织，突触可塑性，血管发育和包括癌症在内的各种疾病的进展。产生促红细胞生成素的肝细胞受体酪氨酸激酶成员EphB6是一种假激酶，与具有酶活性的对应物相比，它没有引起等量的兴趣。这项研究的目的是评估EphB6在垂体瘤中的表达。
结果：使用实时PCR检测人正常垂体和垂体肿瘤的EphB6mRNA表达，并通过免疫组织化学和Western印迹检测EphB6蛋白。与正常垂体和分泌GH的PitNETs相比，EphB6在无功能的垂体神经内分泌肿瘤（NF-PitNETs）中高表达。EphB6mRNA表达与肿瘤大小相关。
结论：我们的结果表明EphB6在NF-PitNETs中异常表达。未来的研究有必要确定EphB6在NF-PitNETs肿瘤发生中的作用和意义。
结果：使用实时PCR检测人正常垂体和垂体肿瘤的EphB6mRNA表达，并通过免疫组织化学和Western印迹检测EphB6蛋白。与正常垂体和分泌GH的PitNETs相比，EphB6在无功能的垂体神经内分泌肿瘤（NF-PitNETs）中高表达。EphB6mRNA表达与肿瘤大小相关。
结论：我们的结果表明EphB6在NF-PitNETs中异常表达。未来的研究有必要确定EphB6在NF-PitNETs肿瘤发生中的作用和意义。
