Abstract：
Several immune-related genes, including Toll-like receptors (TLR), are associated with circadian rhythms in mammals. However, information on the circadian rhythmic expression of TLRs in fish is limited. In this study, we aimed to analyze the regulation of diel oscillations in the expression of TLR genes in Japanese medaka (Oryzias latipes). The expression analysis revealed diel expression patterns of tlr1, tlr5m, tlr21, and clock genes (bmal1 and clock1) under a 12 h light:12 h dark cycle. The clock gene response element (E-box) was identified in the transcriptional regulatory regions of tlr1, tlr5m, and tlr21. Moreover, overexpressed bmal1 and clock1 enhanced expression levels of tlr1, tlr5m, and tlr21 in medaka embryo (OLHdrR-e3) cells. The expression of tlr1, tlr5m, and tlr21 was significantly decreased in OLHdrR-e3 after generating a bmal1 knockdown using a morpholino oligo. These results indicate the regulation of the diel rhythmic expression of several fish TLRs by clock genes.
摘要：
几个免疫相关基因，包括Toll样受体（TLR），与哺乳动物的昼夜节律有关。然而,有关鱼类TLRs昼夜节律表达的信息有限。在这项研究中,我们旨在分析日本medaka（Oryziaslatipes）中TLR基因表达中diel振荡的调节。表达分析揭示了tlr1、tlr5m、tlr21和时钟基因（bmal1和clock1）在12小时的光照下：12小时的黑暗周期。在tlr1、tlr5m、和tlr21。此外，过表达的bmal1和clock1增强了tlr1，tlr5m的表达水平，和tlr21在medaka胚胎（OLHdrR-e3）细胞中。tlr1、tlr5m、在使用吗啉代寡核苷酸产生bmal1敲低后，OLHdrR-e3中的tlr21显着降低。这些结果表明时钟基因对几种鱼类TLR的diel节律表达的调节。
