Abstract：
The possibility of controlling periorbital hyperpigmentation disorders is one of the most important research goals in cosmetic preparations. In the current investigation, 1% vitamin K (Vit K) was incorporated into a Chitosan/alginate hydrogel which aimed to increase the dermal delivery and anti-pigmentation effect. The Vit K-hydrogel was evaluated using several different tests, including volume expansion/contraction analysis, differential scanning calorimetry (DSC), scanning electron microscopy (SEM), ultraviolet (UV) absorbance spectroscopy, and attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy. Vit K hydrogel\'s drug release profile showed a steady increase over time. Furthermore, the modified Vit K hydrogel formulations showed no harmful effects in an in vitro cytotoxicity study. The Vit K hydrogel was tested for dermal irritation on Wistar rats, and the hydrogel was found to be non-irritating. Furthermore, Vit K-hydrogel inhibited melanin formation (31.76 ± 1.14%) and was remarkably higher than free Vit K. In addition, Vit K-hydrogel inhibited L-dopa auto-oxidation to a greater extent (94.80 ± 2.41%) in comparison with Vit K solution (73.95 ± 1.62%). Vit K-hydrogel enhanced percutaneous transport of Vit K, according to in vitro percutaneous absorption findings, suggesting that this innovative formulation may provide new therapeutic options for periorbital hyperpigmentation.
摘要：
控制眶周色素沉着障碍的可能性是化妆品制剂中最重要的研究目标之一。在目前的调查中,将1%维生素K(VitK)掺入到壳聚糖/藻酸盐水凝胶中,其旨在增加皮肤递送和抗色素沉着效果。使用几种不同的测试评估了VitK水凝胶，包括体积膨胀/收缩分析，差示扫描量热法(DSC),扫描电子显微镜（SEM），紫外（UV）吸收光谱，和衰减全反射傅里叶变换红外(ATR-FTIR)光谱。VitK水凝胶的药物释放曲线显示出随时间的稳定增加。此外，改性的VitK水凝胶制剂在体外细胞毒性研究中没有显示出有害作用。测试了VitK水凝胶对Wistar大鼠的皮肤刺激，并且发现水凝胶是无刺激性的。此外，维生素K-水凝胶抑制黑色素形成(31.76±1.14%),显著高于游离维生素K。与VitK溶液（73.95±1.62％）相比，VitK水凝胶在更大程度上抑制了L-多巴自动氧化（94.80±2.41％）。维生素K-水凝胶增强维生素K的经皮转运，根据体外经皮吸收结果，提示这种创新的配方可能为眶周色素沉着提供新的治疗选择.
