PDF(Pubmed)
Abstract：
Growth is a fundamental aspect of aquaculture breeding programs, pivotal for successful cultivation. Understanding the mechanisms that govern growth and development differences across various stages can significantly boost seedling production of economically valuable species, thereby enhancing aquaculture efficiency and advancing the aquaculture industry. Mytilus coruscus, a commercially vital marine bivalve, underscores this importance. To decipher the intricate molecular mechanisms dictating growth and developmental disparities in marine shellfish, we conducted transcriptome sequencing and meticulously analyzed gene expression variations and molecular pathways linked to growth traits in M. coruscus. This study delved into the molecular and gene expression variations across five larval development stages, with a specific focus on scrutinizing the differential expression patterns of growth-associated genes using RNA sequencing and quantitative real-time PCR analysis. A substantial number of genes-36,044 differentially expressed genes (DEGs)-exhibited significant differential expression between consecutive developmental stages. These DEGs were then categorized into multiple pathways (Q value < 0.05), including crucial pathways such as the spliceosome, vascular smooth muscle contraction, DNA replication, and apoptosis, among others. In addition, we identified two pivotal signaling pathways-the Hedgehog (Hh) signaling pathway and the TGF-beta (TGF-β) signaling pathway-associated with the growth and development of M. coruscus larvae. Ten key growth-related genes were pinpointed, each playing crucial roles in molecular function and the regulation of growth traits in M. coruscus. These genes and pathways associated with growth provide deep insights into the molecular basis of physiological adaptation, metabolic processes, and growth variability in marine bivalves.
摘要：
生长是水产养殖计划的一个基本方面，成功种植的关键。了解控制各个阶段生长和发育差异的机制可以显着提高有经济价值的物种的幼苗产量，从而提高水产养殖效率，促进水产养殖业的发展。Mytiluscoruscus,具有商业意义的海洋双壳类动物，强调了这一重要性。为了破译决定海洋贝类生长和发育差异的复杂分子机制，我们进行了转录组测序，并仔细分析了与M.coruscus生长性状相关的基因表达变异和分子途径。本研究深入研究了五个幼虫发育阶段的分子和基因表达变异，特别关注使用RNA测序和定量实时PCR分析审查生长相关基因的差异表达模式。大量基因-36,044个差异表达的基因（DEG）-在连续的发育阶段之间表现出明显的差异表达。然后将这些DEG分类为多个途径(Q值<0.05)，包括关键途径，如剪接体，血管平滑肌收缩，DNA复制,和细胞凋亡，在其他人中。此外,我们确定了两个关键的信号通路-Hedgehog（Hh）信号通路和TGF-β（TGF-β）信号通路-与M的生长和发育有关。确定了10个关键的生长相关基因，每个都在M的分子功能和生长性状的调节中起着至关重要的作用。这些与生长相关的基因和途径为生理适应的分子基础提供了深刻的见解，代谢过程，和海洋双壳类动物的生长变异性。
