PDF(Pubmed)
Abstract：
The study investigated a profile of chemokines and growth factors in the aqueous humor (AH) of eyes with Fuch\'s endothelial corneal dystrophy (FECD) and cataracts in comparison with cataract patients as a control group. A total of 52 AH samples (26 FECD + cataract and 26 cataract/control) were collected before cataract surgery. None of the patients had any clinically apparent inflammation at the time of AH collection. The AH levels of MCP-1 (CCL2), MIP-1α (CCL3), MIP-1β(CCL4), RANTES (CCL5), eotaxin (CCL11), IP-10 (CXCL10), FGF basic, G-CSF, GM-CSF, PDGF-bb, and VEGF were compared between the groups. The analyses were performed using the Bio-Plex 200 System from Bio-Rad. Among the studied parameters, the AH levels of RANTES, eotaxin, and IP-10 significantly increased in the FECD + cataract eyes, compared with the cataract controls (p < 0.05). Elevated levels of the RANTES, Eotaxin, and IP-10 indicate more intense inflammation in the eyes of patients in the FECD + cataract group. Moreover, these factors exhibit potential as predictive biomarkers for early detection of FECD in cataract patients. The discovery of elevated concentrations of biochemical markers in a patient, who has not yet received a clinical diagnosis, may suggest the need for heightened observation of the other eye to monitor the potential development of FECD.
摘要：
该研究调查了Fuch's内皮角膜营养不良（FECD）和白内障患者眼房水（AH）中趋化因子和生长因子的分布，并与对照组的白内障患者进行了比较。在白内障手术前收集总共52个AH样品(26个FECD+白内障和26个白内障/对照)。在AH收集时,没有患者具有任何临床上明显的炎症。MCP-1（CCL2）的AH水平，MIP-1α(CCL3),MIP-1β(CCL4),RANTES(CCL5),eotaxin（CCL11），IP-10(CXCL10)，FGF基础，G-CSF,GM-CSF,PDGF-bb,组间比较VEGF。使用来自Bio-Rad的Bio-Plex200系统进行分析。在研究的参数中，RANTES的AH等级,eotaxin,在FECD+白内障眼中IP-10显著增加，与白内障对照组相比(p<0.05)。RANTES的水平升高，eotaxin,和IP-10表明FECD+白内障组患者的眼部炎症更强烈。此外，这些因素有可能作为白内障患者早期检测FECD的预测生物标志物.发现患者体内生化标志物浓度升高，尚未接受临床诊断的人，可能建议需要加强对另一只眼睛的观察，以监测FECD的潜在发展。
