PDF(Pubmed)
Abstract：
Annexins (ANNs) are a structurally conserved protein family present in almost all plants. In the present study, 27 GhANNs were identified in cotton and were unevenly distributed across 14 chromosomes. Transcriptome data and RT-qPCR results revealed that multiple GhANNs respond to at least two abiotic stresses. Similarly, the expression levels of GhANN4 and GhANN11 were significantly upregulated under heat, cold, and drought stress. Using virus-induced gene silencing (VIGS), functional characterization of GhANN4 and GhANN11 revealed that, compared with those of the controls, the leaf wilting of GhANN4-silenced plants was more obvious, and the activities of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) were lower under NaCl and PEG stress. Moreover, the expression of stress marker genes (GhCBL3, GhDREB2A, GhDREB2C, GhPP2C, GhRD20-2, GhCIPK6, GhNHX1, GhRD20-1, GhSOS1, GhSOS2 and GhSnRK2.6) was significantly downregulated in GhANN4-silenced plants after stress. Under cold stress, the growth of the GHANN11-silenced plants was significantly weaker than that of the control plants, and the activities of POD, SOD, and CAT were also lower. However, compared with those of the control, the elasticity and orthostatic activity of the GhANN11-silenced plants were greater; the POD, SOD, and CAT activities were higher; and the GhDREB2C, GhHSP, and GhSOS2 expression levels were greater under heat stress. These results suggest that different GhANN family members respond differently to different types of abiotic stress.
摘要：
膜联蛋白(ANN)是存在于几乎所有植物中的结构保守的蛋白质家族。在本研究中,在棉花中鉴定出27个GhANN，它们在14条染色体上分布不均匀。转录组数据和RT-qPCR结果表明，多个GhANN响应至少两种非生物胁迫。同样，GhANN4和GhANN11的表达水平在高温下显著上调，冷,和干旱胁迫。使用病毒诱导的基因沉默（VIGS），GhANN4和GhANN11的功能表征表明，与对照组相比，GhANN4沉默植物的叶片枯萎更为明显，和过氧化氢酶（CAT）的活性，过氧化物酶(POD),在NaCl和PEG胁迫下，超氧化物歧化酶（SOD）降低。此外，应激标记基因的表达(GhCBL3，GhDREB2A，GhDREB2C,GhPP2C,GhRD20-2，GhCIPK6，GhNHX1，GhRD20-1，GhSOS1，GhSOS2和GhSnRK2.6）在胁迫后在GhANN4沉默的植物中被显着下调。在寒冷的压力下，GHANN11沉默植物的生长明显弱于对照植物，以及POD的活动，SOD,CAT也较低。然而,与对照组相比，GhANN11沉默植物的弹性和直立活性更大；POD，SOD,CAT活性更高；GhDREB2C，GhHSP,热应激下GhSOS2表达水平更高。这些结果表明，不同的GhANN家族成员对不同类型的非生物胁迫的反应不同。
