PDF(Pubmed)
Abstract：
CTP synthase (CTPS), the rate-limiting enzyme in the de novo synthesis of CTP, assembles into a filamentous structure termed the cytoophidium. The Hippo pathway regulates cell proliferation and apoptosis. The relationship of the nucleotide metabolism with the Hippo pathway is little known. Here, we study the impact of the Hippo pathway on the cytoophidium in Drosophila melanogaster posterior follicle cells (PFCs). We find that the inactivation of the Hippo pathway correlates with reduced cytoophidium length and number within PFCs. During the overexpression of CTPS, the presence of Hippo mutations also reduces the length of cytoophidia in PFCs. In addition, we observe that knocking down CTPS mitigates hpo (Hippo)-associated over-proliferation. In summary, our results suggest that there is a connection between the Hippo pathway and the nucleotide biosynthesis enzyme CTPS in PFCs.
摘要：
CTP合酶(CTPS),从头合成CTP的限速酶，组装成一种称为胞质的丝状结构。Hippo通路调节细胞增殖和凋亡。核苷酸代谢与Hippo途径的关系鲜为人知。这里,我们研究了Hippo通路对果蝇后滤泡细胞（PFCs）的影响。我们发现，Hippo途径的失活与PFCs内胞质长度和数量减少有关。在CTPS的过表达过程中,Hippo突变的存在也减少了PFCs中胞质的长度。此外,我们观察到，敲低CTPS可减轻hpo（Hippo）相关的过度增殖。总之,我们的结果表明，在PFCs中，Hippo途径与核苷酸生物合成酶CTPS之间存在联系。
