PDF(Pubmed)
Abstract：
To limit the spread of bovine ringworm, control measures such as movement restrictions are highly recommended. In this context, calves at auction markets in Styria, Austria, displaying skin lesions characteristic for bovine ringworm, are excluded from the auctions. To investigate whether these clinical assessments correspond to laboratory diagnosis, a total of 166 samples taken from skin lesions assigned to the three clinical categories \'ringworm very likely (v), likely (l) or unlikely (u)\' were mycologically examined using microscopy, culture, and nested PCR followed by amplicon sequencing. Further, the relationships of isolated dermatophytes were determined through multi-locus sequence typing (MLST). Overall, a high agreement between clinical assessment and laboratory results were observed with microscopy and nested PCR, providing more consistent results and molecular detection possessing an analytical sensitivity superior to that of cultural isolation (culture 21.7% vs. nested PCR 48.2%). Phylogenetic analyses revealed that most of the isolated dermatophytes belong to a unique Trichophyton verrucosum MLST genotype. In conclusion, clinical assessments were largely confirmed through laboratory diagnosis with nested PCR and sequencing, providing rapid, sensitive, and species-specific detection of dermatophytes in calves at auction markets displaying skin lesions typical for ringworm; this seems to be predominantly caused by a single Trichophyton verrucosum strain.
摘要：
为了限制牛癣的传播，强烈建议采取控制措施，如移动限制。在这种情况下，施蒂里亚州拍卖市场上的小牛，奥地利,显示牛癣的皮肤病变特征，被排除在拍卖之外。为了调查这些临床评估是否符合实验室诊断，总共166个样本取自皮肤损伤，这三个临床类别很可能是癣（v），可能(l)或不太可能(u)用显微镜检查真菌学，文化,和巢式PCR，然后进行扩增子测序。Further,通过多位点序列分型（MLST）确定分离的皮肤癣菌的关系。总的来说,用显微镜和巢式PCR观察到临床评估和实验室结果之间的高度一致性，提供更一致的结果和分子检测，具有优于文化隔离的分析灵敏度(文化21.7%vs.巢式PCR48.2%)。系统发育分析表明，大多数分离的皮肤癣菌属于独特的疣毛癣菌MLST基因型。总之,临床评估主要通过巢式PCR和测序的实验室诊断得到证实，提供快速，敏感,在拍卖市场上对小牛中的皮肤癣菌进行物种特异性检测，显示出典型的癣皮损；这似乎主要是由单个疣毛癣菌菌株引起的。
