PDF(Pubmed)
Abstract：
Our previous work in guinea pigs revealed that low vitamin C intake during preconception and pregnancy adversely affects fertility, pregnancy outcomes, and foetal and neonatal growth in a sex-dependent manner. To investigate the long-term impact on offspring, we monitored their growth from birth to adolescence (four months), recorded organ weights at childhood equivalence (28 days) and adolescence, and assessed physiological parameters like oral glucose tolerance and basal cortisol concentrations. We also investigated the effects of the timing of maternal vitamin C restriction (early vs. late gestation) on pregnancy outcomes and the health consequences for offspring. Dunkin Hartley guinea pigs were fed an optimal (900 mg/kg feed) or low (100 mg/kg feed) vitamin C diet ad libitum during preconception. Pregnant dams were then randomised into four feeding regimens: consistently optimal, consistently low, low during early pregnancy, or low during late pregnancy. We found that low maternal vitamin C intake during early pregnancy accelerated foetal and neonatal growth in female offspring and altered glucose homeostasis in the offspring of both sexes at an age equivalent to early childhood. Conversely, low maternal vitamin C intake during late pregnancy resulted in foetal growth restriction and reduced weight gain in male offspring throughout their lifespan. We conclude that altered vitamin C during development has long-lasting, sex-specific consequences for offspring and that the timing of vitamin C depletion is also critical, with low levels during early development being associated with the development of a metabolic syndrome-related phenotype, while later deprivation appears to be linked to a growth-faltering phenotype.
摘要：
我们以前在豚鼠中的研究表明，在孕前和怀孕期间维生素C的低摄入量会对生育能力产生不利影响。妊娠结局,胎儿和新生儿以性别依赖的方式生长。为了调查对后代的长期影响，我们监测了他们从出生到青春期（四个月）的成长，记录在儿童期（28天）和青春期的器官重量，并评估生理参数，如口服葡萄糖耐量和基础皮质醇浓度。我们还调查了母体维生素C限制时机的影响(早期与妊娠晚期）对妊娠结局和后代的健康后果。DunkinHartley豚鼠在孕前随意饲喂最佳（900mg/kg饲料）或低（100mg/kg饲料）维生素C饮食。然后将怀孕的水坝随机分为四种喂养方案：始终最佳，持续低,在怀孕早期低，或低在怀孕后期。我们发现，怀孕早期母体维生素C的低摄入量会加速雌性后代的胎儿和新生儿的生长，并改变两性后代的葡萄糖稳态，其年龄与幼儿期相同。相反，妊娠晚期母体维生素C摄入量低导致胎儿生长受限,男性后代在整个生命周期中体重增加减少.我们得出的结论是，在发育过程中改变维生素C具有持久的作用，对后代的性别特异性后果以及维生素C消耗的时机也至关重要，在早期发育过程中低水平与代谢综合征相关表型的发展有关，而后来的剥夺似乎与生长迟缓的表型有关。
