Abstract：
We designed a multi-modal biosensing platform for versatile detection of penicillin based on a unique Ag-ZnIn2S4@Ag-Pt signal probe-sensitized UiO-66 metal-organic framework. Firstly, a large number of Ag-ZnIn2S4 quantum dots (AZIS QDs) were attached to Ag-Pt NPs, preparing a new multi-signal probe AZIS QDs@Ag-Pt NPs with excellent photoelectrochemistry (PEC), electrochemiluminescence (ECL), and fluorescence (FL) signals. Moreover, the AZIS QDs@Ag-Pt NPs signal probe can well match the energy level of UiO-66 metal-organic framework (MOF) with good photoelectric property, which can reverse the PEC current of UiO-66 to reduce false positives in detection. When penicillin was present, it bound to its aptamer to release the multifunctional signal probes, which can generate PEC, ECL, and PL signals, thus realizing ultrasensitive detection of penicillin by multi-signals. This work creates a novel three-signal QDs probe, which makes a great contribution to multi-mode photoelectric sensing analysis. The LOD of this work (3.48 fg·mL-1) was much lower than the MRLs (Maximum Residue Levels) established by the EU (4 ng·mL-1). The newly developed multi-mode biosensor has good practical application values in various biological detection, food assay, and early disease diagnosis.
摘要：
我们基于独特的Ag-ZnIn2S4@Ag-Pt信号探针敏化的UiO-66金属有机框架，设计了一种用于通用检测青霉素的多模态生物传感平台。首先,大量的Ag-ZnIn2S4量子点（AZISQDs）附着在Ag-PtNPs上，制备新型多信号探针AZISQDs@Ag-PtNPs,具有优异的光电化学性能(PEC),电化学发光(ECL)，和荧光(FL)信号。此外，AZISQDs@Ag-PtNPs信号探针能很好地匹配UiO-66金属有机骨架(MOF)的能级,具有良好的光电性能,这可以逆转UiO-66的PEC电流以减少检测中的假阳性。当青霉素存在时，它与它的适体结合以释放多功能信号探针，可以产生PEC，ECL,和PL信号，从而实现多信号对青霉素的超灵敏检测。这项工作创造了一种新颖的三信号量子点探针，这对多模式光电传感分析有很大的贡献。这项工作的LOD（3.48fg·mL-1）远低于EU建立的MRL（最大残留水平）（4ng·mL-1）。新研制的多模生物传感器在各种生物检测中具有良好的实际应用价值,食物化验,和早期疾病诊断。
