PDF(Pubmed)
Abstract：
For Raman hyperspectral detection and imaging in live cells, it is very desirable to create novel probes with strong and unique Raman vibrations in the biological silent region (1800-2800 cm-1). The use of molecular probes in Raman imaging is a relatively new technique in subcellular research; however, it is developing very rapidly. Compared with the label-free method, it allows for a more sensitive and selective visualization of organelles within a single cell. Biological systems are incredibly complex and heterogeneous. Directly visualizing biological structures and activities at the cellular and subcellular levels remains by far one of the most intuitive and powerful ways to study biological problems. Each organelle plays a specific and essential role in cellular processes, but importantly for cells to survive, mitochondrial function must be reliable. Motivated by earlier attempts and successes of biorthogonal chemical imaging, we develop a tool supporting Raman imaging of cells to track biochemical changes associated with mitochondrial function at the cellular level in an in vitro model. In this work, we present a newly synthesized highly sensitive RAR-BR Raman probe for the selective imaging of mitochondria in live endothelial cells.
摘要：
用于活细胞中的拉曼高光谱检测和成像，非常希望在生物沉默区域(1800-2800cm-1)中产生具有强且独特的拉曼振动的新型探针。分子探针在拉曼成像中的使用是亚细胞研究中相对较新的技术；然而，它发展非常迅速。与无标签方法相比，它允许单个细胞内的细胞器更灵敏和选择性的可视化。生物系统非常复杂和异构。在细胞和亚细胞水平上直接可视化生物结构和活动仍然是研究生物学问题的最直观和最有力的方法之一。每个细胞器在细胞过程中都发挥着特定和重要的作用，但重要的是细胞存活，线粒体功能必须可靠。受到早期双正交化学成像的尝试和成功的激励，我们开发了一种支持细胞拉曼成像的工具，以在体外模型中跟踪细胞水平上与线粒体功能相关的生化变化。在这项工作中,我们提出了一种新合成的高灵敏度RAR-BR拉曼探针，用于活内皮细胞线粒体的选择性成像。
