PDF(Pubmed)
Abstract：
The growth of liquid biopsy, i. e., the possibility of obtaining health information by analysing circulating species (nucleic acids, cells, proteins, and vesicles) in peripheric biofluids, is pushing the field of sensors and biosensors beyond the limit to provide decentralised solutions for nonspecialists. In particular, among all the circulating species that can be adopted in managing cancer evolution, both for diagnostic and prognostic applications, microRNAs have been highly studied and detected. The development of electrochemical devices is particularly relevant for liquid biopsy purposes, and the screen-printed electrodes (SPEs) represent one of the building blocks for producing novel portable devices. In this work, we have taken miR-2115-3p as model target (it is related to lung cancer), and we have developed a biosensor by exploiting the use of a complementary DNA probe modified with methylene blue as redox mediator. In particular, the chosen sensing architecture was applied to serum measurements of the selected miRNA, obtaining a detection limit within the low nanomolar range; in addition, various platforms were interrogated, namely commercial and hand-made SPEs, with the aim of providing the reader with some insights about the optimal platform to be used by considering both the cost and the analytical performance.
摘要：
液体活检的生长，i.e.,通过分析循环物种(核酸，细胞,蛋白质，和囊泡）在外周生物流体中，正在推动传感器和生物传感器领域超越极限，为非专业人士提供分散的解决方案。特别是,在所有可用于管理癌症进化的循环物种中，用于诊断和预后应用，microRNAs已被高度研究和检测。电化学装置的发展与液体活检目的特别相关，和丝网印刷电极(SPE)代表用于生产新型便携式设备的构件之一。在这项工作中,我们将miR-2115-3p作为模型靶标（它与肺癌有关），我们通过使用亚甲基蓝修饰的互补DNA探针作为氧化还原介体，开发了一种生物传感器。特别是,将所选的传感结构应用于所选miRNA的血清测量，在低纳摩尔范围内获得检测极限；此外，各种平台被审问，即商业和手工制作的SPE，目的是通过考虑成本和分析性能，为读者提供有关要使用的最佳平台的一些见解。
