PDF(Pubmed)
Abstract：
Acetylcarnitine is an essential metabolite for maintaining metabolic flexibility and glucose homeostasis. The in vivo behavior of muscle acetylcarnitine content during exercise has not been shown with magnetic resonance spectroscopy. Therefore, this study aimed to explore the behavior of skeletal muscle acetylcarnitine during rest, plantar flexion exercise, and recovery in the human gastrocnemius muscle under aerobic conditions. Ten lean volunteers and nine overweight volunteers participated in the study. A 7 T whole-body MR system with a double-tuned surface coil was used to acquire spectra from the gastrocnemius medialis. An MR-compatible ergometer was used for the plantar flexion exercise. Semi-LASER-localized 1H MR spectra and slab-localized 31P MR spectra were acquired simultaneously in one interleaved exercise/recovery session. The time-resolved interleaved 1H/31P MRS acquisition yielded excellent data quality. A between-group difference in acetylcarnitine metabolism over time was detected. Significantly slower τPCr recovery, τPCr on-kinetics, and lower Qmax in the overweight group, compared to the lean group was found. Linear relations between τPCr on-kinetics, τPCr recovery, VO2max and acetylcarnitine content were identified. In conclusion, we are the first to show in vivo changes of skeletal muscle acetylcarnitine during acute exercise and immediate exercise recovery with a submaximal aerobic workload using interleaved 1H/31P MRS at 7 T.
摘要：
乙酰肉碱是维持代谢灵活性和葡萄糖稳态的必需代谢产物。磁共振波谱未显示运动过程中肌肉乙酰肉碱含量的体内行为。因此,本研究旨在探讨骨骼肌乙酰肉碱在休息时的行为，足底屈曲运动,在有氧条件下恢复人腓肠肌。10名瘦志愿者和9名超重志愿者参与了这项研究。使用具有双调谐表面线圈的7T全身MR系统从腓肠肌中获取光谱。MR兼容的测力计用于足底屈曲运动。在一个交错的运动/恢复会话中同时采集半激光定位的1HMR光谱和平板定位的31PMR光谱。时间分辨交错的1H/31PMRS采集产生了出色的数据质量。检测到乙酰肉碱代谢随时间的组间差异。τPCr恢复明显较慢，τPCr导通动力学，超重组的Qmax较低，与精益组相比，发现。τPCr对动力学之间的线性关系，τPCr恢复，确定了VO2max和乙酰肉碱含量。总之,我们首次在7T时使用交错的1H/31PMRS在急性运动和立即运动恢复过程中显示骨骼肌乙酰肉碱的体内变化。
