RESULTS: EMS mutagenesis had a substantial effect on banana seedlings, resulting in modifications in shoot and root traits, wherein a majority of seedlings exhibited delayed differentiation and limited elongation. Notably, mutant leaves displayed altered biomass composition, with starch content exhibiting the most pronounced variation. The application of L-Hyp pressure selection aided in the identification of cold-resistant mutants among seedling-lethal phenotypes. The mutant cm784 demonstrated enhanced cold resistance, as evidenced by improved survival rates and reduced symptoms of chilling injury. Physiological analyses demonstrated heightened activities of antioxidant enzymes and increased proline production in cm784 when subjected to cold stress. Transcriptome analysis unveiled 946 genes that were differentially expressed in cm784, with a notable enrichment in categories related to \'Carbohydrate transport and metabolism\' and \'Secondary metabolites biosynthesis, transport, and catabolism\'.
CONCLUSIONS: The present findings provide insights into the molecular mechanisms that contribute to the heightened cold resistance observed in banana mutants. These mechanisms encompass enhanced carbohydrate metabolism and secondary metabolite biosynthesis, thereby emphasizing the adaptive strategies employed to mitigate the detrimental effects induced by cold stress.
结果:EMS诱变对香蕉幼苗有很大影响,导致芽和根性状的改变,其中大多数幼苗表现出延迟的分化和有限的伸长。值得注意的是,突变叶显示出改变的生物量组成,淀粉含量表现出最明显的变化。L-Hyp压力选择的应用有助于鉴定幼苗致死表型中的耐寒突变体。突变体cm784表现出增强的抗寒性,如提高生存率和减少冷害症状所证明的。生理分析表明,当遭受冷胁迫时,cm784中的抗氧化酶活性增强,脯氨酸产量增加。转录组分析揭示了在cm784中差异表达的946个基因,在与“碳水化合物运输和代谢”和“次级代谢产物生物合成”相关的类别中具有显着的富集,运输,和分解代谢。
结论:目前的发现提供了对分子机制的见解,这些分子机制有助于在香蕉突变体中观察到增强的抗寒性。这些机制包括增强碳水化合物代谢和次级代谢产物生物合成,从而强调了用于减轻冷应力引起的有害影响的适应性策略。