Abstract：
The phenotype of allergic diseases associated with Anisakis determines the pattern of cytokines related to antibody production. However, the role of serum IgA and the immunomodulatory mechanisms exerted by active infection of L3 or passive mucosal contact with A. simplex specific antigens has not been studied before. We measured serum cytokine by flow cytometry (IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ, IL-17A, TGF-β1) and antibody levels (IgE, IgG4, IgA) by ELISA against total and excretory-secretory (ES) antigens, Ani s 3,and the group of major allergens Ani s 1, Ani s 7, and Ani s 13 in sera from 10 patients with gastro-allergic anisakiasis (GAA), 11 Anisakis sensitization associated chronic urticaria (CU+) as well as 17 non-Anisakis-sensitized patients with chronic urticaria (CU-), compared with the urticaria control group (18 subjects). Specific IgE, IgG4 and IgA were high in the GAA, but IgA levels were significantly higher in the CU+ with respect the CONTROL group. We observed higher levels of the ratio IgA/IgG4 in CU+ than GAA group for Ani s 1, Ani s 7, Ani s 13 and ES. Furthermore, chronic urticaria (CU) patients showed significant lower levels of IL-10, IFN-γ and IL-17A than patients without CU. The anti-Ani s 13 IgA/IgG4 ratio correlated positively with pro-inflammatory cytokines and ratios (TNF-α, IL-17A, Th17/Th2, Type1/Type2 and TNF-α/IL-10) in CONTROL group. In general, Anti-Anisakis IgA/G4 ratio was high in CU patients. In conclusion, this study demonstrates the importance of serum IgA because it is associated with chronic urticaria independently of Anisakis sensitization.
摘要：
与Anisakis相关的过敏性疾病的表型决定了与抗体产生相关的细胞因子的模式。然而,血清IgA的作用以及主动感染L3或被动粘膜接触A.我们通过流式细胞术测量血清细胞因子(IL-2，IL-4，IL-6，IL-10，TNF-α，IFN-γ，IL-17A,TGF-β1）和抗体水平（IgE，IgG4，IgA）通过ELISA针对总和排泄分泌（ES）抗原，来自10例胃过敏性anisakiasis（GAA）患者的血清中的Anis3和主要过敏原Anis1，Anis7和Anis13，11Anisakis致敏相关的慢性荨麻疹（CU）以及17例非Anisakis致敏的慢性荨麻疹（CU-）患者，与荨麻疹对照组(18例)比较。特异性IgE,IgG4和IgA在GAA中很高，但与对照组相比，CU+组的IgA水平明显更高。对于Anis1，Anis7，Anis13和ES，我们观察到CU中的IgA/IgG4比值高于GAA组。此外，慢性荨麻疹（CU）患者的IL-10，IFN-γ和IL-17A水平明显低于无CU患者。抗Anis13IgA/IgG4比值与促炎细胞因子和比值呈正相关(TNF-α，IL-17A,对照组Th17/Th2、Type1/Type2和TNF-α/IL-10)。总的来说,CU患者的抗异尖异语系IgA/G4比值较高。总之,这项研究证明了血清IgA的重要性，因为它与慢性荨麻疹相关，而与Anisakis致敏无关。
