PDF(Pubmed)
Abstract：
ALG3 has significant modulatory function in the process of tumor development. Yet how ALG3 involves in the advancement of different malignancies isn\'t fully understood. We performed a pan-cancer assessment on ALG3 utilizing datasets from The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) to examine its tumor-related roles across malignancies and its link to particular molecules and cells in the tumor microenvironment (TME). Furthermore, we focused on breast cancer to examine the influence of ALG3-mediated signaling pathways and intercellular interactions in the advancement of tumors. The biological effects of ALG3 were verified by breast cancer cells. Enhanced ALG3 expression was discovered to be substantially linked to patients\' grim prognoses in a number of malignancies. Furthermore, the expression of ALG3 in the TME was linked to the infiltration of stromal and immune cells, and ALG3-related immune checkpoints, TMB, and MSI were also discovered. We also discovered that cancer patients having a high level of ALG3 exhibited a lower probability of benefiting from immunotherapy. Furthermore, our research found that KEGG enrichment, single-cell RNA and spatial sequencing analyses were effective in identifying key signaling pathways in ALG3-associated tumor growth. In vitro, knockdown of ALG3 could decrease the proliferation of breast cancer cells. In summary, our research offers a comprehensive insight into the advancement of tumors under the mediation of ALG3. ALG3 appears to be intimately associated with tumor development in the TME. ALG3 might be a viable treatment target for cancer therapy, particularly in the case of breast cancer.
摘要：
ALG3在肿瘤发展进程中具有显著的调控感化。然而，ALG3如何参与不同恶性肿瘤的发展还没有完全理解。我们利用来自癌症基因组图谱（TCGA）和基因型组织表达（GTEx）的数据集对ALG3进行了泛癌症评估，以检查其在恶性肿瘤中的肿瘤相关作用及其与肿瘤微环境中特定分子和细胞的联系（TME）。此外，我们将重点放在乳腺癌上,以研究ALG3介导的信号通路和细胞间相互作用在肿瘤进展中的影响.ALG3的生物学效应在乳腺癌细胞中得到验证。发现增强的ALG3表达与许多恶性肿瘤患者的严峻预后密切相关。此外，ALG3在TME中的表达与基质细胞和免疫细胞的浸润有关，和ALG3相关的免疫检查点，TMB,MSI也被发现了。我们还发现,具有高水平ALG3的癌症患者表现出较低的受益于免疫疗法的可能性。此外，我们的研究发现KEGG富集，单细胞RNA和空间测序分析可有效识别ALG3相关肿瘤生长的关键信号通路.体外，ALG3敲除可以降低乳腺癌细胞的增殖。总之,我们的研究为ALG3介导下肿瘤的进展提供了全面的见解.ALG3似乎与TME中的肿瘤发展密切相关。ALG3可能是癌症治疗的可行治疗靶点，特别是在乳腺癌的情况下。
