Abstract：
BACKGROUND: Non-nutritive sweeteners (NNS) are commonly used in sweetened foods and beverages; however their role in metabolic regulation is still not clear. In this experiment, we used guinea pigs as an animal model to study the effect of NNS on body growth and intestinal health by modifying gut microbiota and hypothalamus-related proteins.
RESULTS: For a 28-day feeding experiment a total of 40 guinea pigs were randomly divided into four groups, one control (CN) group and three treatments, in which three NNS were added to the diet: rebaudioside A (RA, 330 mg kg-1), sodium saccharin (SS, 800 mg kg-1), and sucralose (TGS, 167 mg kg-1), respectively. The TGS group exhibited significantly reduced food consumption in comparison with the CN group (P < 0.05) whereas the RA group showed increased food consumption in comparison with the CN group (P < 0.05). Notably, Taste receptor type 1 subunit 2 (T1R2) expression in the hypothalamus was significantly higher in the RA group than in the CN group (P < 0.05). The mRNA expressions of appetite-stimulated genes arouti-related neuropeptide (AGRP), neuropeptide Y (NPY), and thyroid stimulating hormone (TSHB) were significantly higher than those in the CN group (P < 0.05) but mRNA expressions of appetite-suppressed genes tryptophan hydroxylase 2(THP2) were significantly lower in the TGS group (P < 0.05). Furthermore, NNS in the guinea pig diets (RA, SS, TGS) significantly increased the relative abundance of Muribaculaceae but decreased the relative abundance of Clostridia_vadin BB60 in comparison with the CN group (P < 0.05). We also found that dietary supplementation with RA also significantly altered the relative abundance of Lactobacillus.
CONCLUSIONS: Our finding confirmed that dietary supplementation with RA and TGS affected body growth and intestinal health by modulating hypothalamic RNA profiles and ileum microbiota, suggesting that NNS should be included in guinea-pig feeding. © 2024 Society of Chemical Industry.
RESULTS: For a 28-day feeding experiment a total of 40 guinea pigs were randomly divided into four groups, one control (CN) group and three treatments, in which three NNS were added to the diet: rebaudioside A (RA, 330 mg kg-1), sodium saccharin (SS, 800 mg kg-1), and sucralose (TGS, 167 mg kg-1), respectively. The TGS group exhibited significantly reduced food consumption in comparison with the CN group (P < 0.05) whereas the RA group showed increased food consumption in comparison with the CN group (P < 0.05). Notably, Taste receptor type 1 subunit 2 (T1R2) expression in the hypothalamus was significantly higher in the RA group than in the CN group (P < 0.05). The mRNA expressions of appetite-stimulated genes arouti-related neuropeptide (AGRP), neuropeptide Y (NPY), and thyroid stimulating hormone (TSHB) were significantly higher than those in the CN group (P < 0.05) but mRNA expressions of appetite-suppressed genes tryptophan hydroxylase 2(THP2) were significantly lower in the TGS group (P < 0.05). Furthermore, NNS in the guinea pig diets (RA, SS, TGS) significantly increased the relative abundance of Muribaculaceae but decreased the relative abundance of Clostridia_vadin BB60 in comparison with the CN group (P < 0.05). We also found that dietary supplementation with RA also significantly altered the relative abundance of Lactobacillus.
CONCLUSIONS: Our finding confirmed that dietary supplementation with RA and TGS affected body growth and intestinal health by modulating hypothalamic RNA profiles and ileum microbiota, suggesting that NNS should be included in guinea-pig feeding. © 2024 Society of Chemical Industry.
摘要：
背景：非营养性甜味剂（NNS）通常用于增甜食品和饮料中；但是它们在代谢调节中的作用尚不清楚。在这个实验中,我们以豚鼠为动物模型,通过修饰肠道菌群和下丘脑相关蛋白,研究NNS对机体生长和肠道健康的影响.
结果：在28天的喂养实验中，将40只豚鼠随机分为四组，一个对照组（CN）和三个治疗，在饮食中添加了三个NNS：莱鲍迪甙A(RA，330mgkg-1)，糖精钠（SS，800mgkg-1)，和三氯蔗糖（TGS，167mgkg-1)，分别。与CN组相比，TGS组的食物消耗量显着降低（P<0.05），而与CN组相比，RA组的食物消耗量增加（P<0.05）。值得注意的是,味觉受体1型亚基2(T1R2)在下丘脑中的表达RA组明显高于CN组(P<0.05)。食欲刺激基因arouti相关神经肽（AGRP）的mRNA表达，神经肽Y（NPY），TGS组食欲抑制基因色氨酸羟化酶2（THP2）的mRNA表达明显低于CN组（P<0.05）。此外，豚鼠饮食中的NNS（RA，SS,与CN组相比，TGS）显着增加了Muribaculaceae的相对丰度，但降低了Clostridia_vadinBB60的相对丰度（P<0.05）。我们还发现，饮食补充RA也显着改变了乳酸菌的相对丰度。
结论：我们的发现证实，RA和TGS的饮食补充通过调节下丘脑RNA谱和回肠菌群影响身体生长和肠道健康，提示NNS应包括在豚鼠喂养中。©2024化学工业学会。
结果：在28天的喂养实验中，将40只豚鼠随机分为四组，一个对照组（CN）和三个治疗，在饮食中添加了三个NNS：莱鲍迪甙A(RA，330mgkg-1)，糖精钠（SS，800mgkg-1)，和三氯蔗糖（TGS，167mgkg-1)，分别。与CN组相比，TGS组的食物消耗量显着降低（P<0.05），而与CN组相比，RA组的食物消耗量增加（P<0.05）。值得注意的是,味觉受体1型亚基2(T1R2)在下丘脑中的表达RA组明显高于CN组(P<0.05)。食欲刺激基因arouti相关神经肽（AGRP）的mRNA表达，神经肽Y（NPY），TGS组食欲抑制基因色氨酸羟化酶2（THP2）的mRNA表达明显低于CN组（P<0.05）。此外，豚鼠饮食中的NNS（RA，SS,与CN组相比，TGS）显着增加了Muribaculaceae的相对丰度，但降低了Clostridia_vadinBB60的相对丰度（P<0.05）。我们还发现，饮食补充RA也显着改变了乳酸菌的相对丰度。
结论：我们的发现证实，RA和TGS的饮食补充通过调节下丘脑RNA谱和回肠菌群影响身体生长和肠道健康，提示NNS应包括在豚鼠喂养中。©2024化学工业学会。
