METHODS: Calcium hydroxide+distilled-water:C, calcium hydroxide+saline:S, calcium hydroxide+synthetic tissue fluid:STF, Dycal:D, calcium hydroxide+distilled-water+RES:C+RES, calcium hydroxide+saline+RES:S+RES, calcium hydroxide+synthetic tissue fluid+RES:STF+RES, Dycal+RES:D+RES were tested. Cytotoxicity was determined by WST-1. Antibacterial-activity was evaluated by agar-diffusion. The water-absorption and solubility were examined for ISO-6876 and ISO-3107. The color-change was evaluated by spectrophotometer. Radiopacity was evaluated for ISO-6876 and ISO-9917. The normal distribution and homogeneity were determined and comparisons were made with appropriate analysis and post hoc tests (P < 0.05).
RESULTS: The highest cell-viability was determined in the C+RES and the lowest was in D and D+RES after 24 h (P < 0.0001). RES-addition increased cell-viability and the highest rate was detected in C+RES, S+RES and STF+RES after 48 h (P < 0.0001). A limited inhibition-zone against Streptococcus mutans was detected in D and D+RES. RES-addition did not change the water-absorption in S and STF or the solubility in S group.
CONCLUSIONS: RES-addition may be used to increase the biocompatibility of calcium hydroxide without any adverse effect on physical properties. Saline may be the first choice as a mixing solution.
方法:氢氧化钙+蒸馏水:C,氢氧化钙+盐水:S,氢氧化钙+合成组织液:STF,Dycal:D,氢氧化钙+蒸馏水+RES:C+RES,氢氧化钙+盐水+RES:S+RES,氢氧化钙+合成组织液+RES:STF+RES,测试Dycal+RES:D+RES。通过WST-1测定细胞毒性。通过琼脂扩散评估抗菌活性。检测ISO-6876和ISO-3107的吸水性和溶解度。通过分光光度计评价颜色变化。对ISO-6876和ISO-9917评估射线不透性。确定正态分布和同质性,并通过适当的分析和事后检验进行比较(P<0.05)。
结果:在24小时后,在CRES中确定最高的细胞活力,在D和DRES中最低(P<0.0001)。RES-添加增加了细胞活力,在C+RES中检测到最高的比率,48h后的S+RES和STF+RES(P<0.0001)。在D和DRES中检测到针对变形链球菌的有限抑制区。RES-添加不改变S和STF中的吸水性或S组中的溶解度。
结论:RES-添加可用于增加氢氧化钙的生物相容性,而对物理性质没有任何不利影响。盐水可以是作为混合溶液的第一选择。