Abstract：
OBJECTIVE: To evaluate the biological and physical properties of calcium hydroxide-containing pulp-capping materials and their modifications with different solutions and antioxidant Resveratrol (RES) addition.
METHODS: Calcium hydroxide+distilled-water:C, calcium hydroxide+saline:S, calcium hydroxide+synthetic tissue fluid:STF, Dycal:D, calcium hydroxide+distilled-water+RES:C+RES, calcium hydroxide+saline+RES:S+RES, calcium hydroxide+synthetic tissue fluid+RES:STF+RES, Dycal+RES:D+RES were tested. Cytotoxicity was determined by WST-1. Antibacterial-activity was evaluated by agar-diffusion. The water-absorption and solubility were examined for ISO-6876 and ISO-3107. The color-change was evaluated by spectrophotometer. Radiopacity was evaluated for ISO-6876 and ISO-9917. The normal distribution and homogeneity were determined and comparisons were made with appropriate analysis and post hoc tests (P < 0.05).
RESULTS: The highest cell-viability was determined in the C+RES and the lowest was in D and D+RES after 24 h (P < 0.0001). RES-addition increased cell-viability and the highest rate was detected in C+RES, S+RES and STF+RES after 48 h (P < 0.0001). A limited inhibition-zone against Streptococcus mutans was detected in D and D+RES. RES-addition did not change the water-absorption in S and STF or the solubility in S group.
CONCLUSIONS: RES-addition may be used to increase the biocompatibility of calcium hydroxide without any adverse effect on physical properties. Saline may be the first choice as a mixing solution.
METHODS: Calcium hydroxide+distilled-water:C, calcium hydroxide+saline:S, calcium hydroxide+synthetic tissue fluid:STF, Dycal:D, calcium hydroxide+distilled-water+RES:C+RES, calcium hydroxide+saline+RES:S+RES, calcium hydroxide+synthetic tissue fluid+RES:STF+RES, Dycal+RES:D+RES were tested. Cytotoxicity was determined by WST-1. Antibacterial-activity was evaluated by agar-diffusion. The water-absorption and solubility were examined for ISO-6876 and ISO-3107. The color-change was evaluated by spectrophotometer. Radiopacity was evaluated for ISO-6876 and ISO-9917. The normal distribution and homogeneity were determined and comparisons were made with appropriate analysis and post hoc tests (P < 0.05).
RESULTS: The highest cell-viability was determined in the C+RES and the lowest was in D and D+RES after 24 h (P < 0.0001). RES-addition increased cell-viability and the highest rate was detected in C+RES, S+RES and STF+RES after 48 h (P < 0.0001). A limited inhibition-zone against Streptococcus mutans was detected in D and D+RES. RES-addition did not change the water-absorption in S and STF or the solubility in S group.
CONCLUSIONS: RES-addition may be used to increase the biocompatibility of calcium hydroxide without any adverse effect on physical properties. Saline may be the first choice as a mixing solution.
摘要：
目的：评估含氢氧化钙的盖髓材料的生物学和物理性质及其在不同溶液和抗氧化剂白藜芦醇（RES）添加下的改性。
方法：氢氧化钙+蒸馏水：C，氢氧化钙+盐水：S，氢氧化钙+合成组织液:STF,Dycal:D,氢氧化钙+蒸馏水+RES:C+RES,氢氧化钙+盐水+RES:S+RES,氢氧化钙+合成组织液+RES:STF+RES,测试Dycal+RES:D+RES。通过WST-1测定细胞毒性。通过琼脂扩散评估抗菌活性。检测ISO-6876和ISO-3107的吸水性和溶解度。通过分光光度计评价颜色变化。对ISO-6876和ISO-9917评估射线不透性。确定正态分布和同质性，并通过适当的分析和事后检验进行比较（P<0.05）。
结果：在24小时后，在CRES中确定最高的细胞活力，在D和DRES中最低（P<0.0001）。RES-添加增加了细胞活力，在C+RES中检测到最高的比率，48h后的S+RES和STF+RES(P<0.0001)。在D和DRES中检测到针对变形链球菌的有限抑制区。RES-添加不改变S和STF中的吸水性或S组中的溶解度。
结论：RES-添加可用于增加氢氧化钙的生物相容性，而对物理性质没有任何不利影响。盐水可以是作为混合溶液的第一选择。
方法：氢氧化钙+蒸馏水：C，氢氧化钙+盐水：S，氢氧化钙+合成组织液:STF,Dycal:D,氢氧化钙+蒸馏水+RES:C+RES,氢氧化钙+盐水+RES:S+RES,氢氧化钙+合成组织液+RES:STF+RES,测试Dycal+RES:D+RES。通过WST-1测定细胞毒性。通过琼脂扩散评估抗菌活性。检测ISO-6876和ISO-3107的吸水性和溶解度。通过分光光度计评价颜色变化。对ISO-6876和ISO-9917评估射线不透性。确定正态分布和同质性，并通过适当的分析和事后检验进行比较（P<0.05）。
结果：在24小时后，在CRES中确定最高的细胞活力，在D和DRES中最低（P<0.0001）。RES-添加增加了细胞活力，在C+RES中检测到最高的比率，48h后的S+RES和STF+RES(P<0.0001)。在D和DRES中检测到针对变形链球菌的有限抑制区。RES-添加不改变S和STF中的吸水性或S组中的溶解度。
结论：RES-添加可用于增加氢氧化钙的生物相容性，而对物理性质没有任何不利影响。盐水可以是作为混合溶液的第一选择。
