Abstract：
UNASSIGNED: Negative pressure wound therapy (NPWT) is considered to be an effective technique to promote the healing of various wounds. The aim of this study was to evaluate different wound dressings combined with NPWT in treating wounds in Wuzhishan pigs.
UNASSIGNED: Excisions were made in the backs of the pigs and were covered with polyvinyl alcohol (PVA) dressing, polyurethane (PU) dressing or PU dressing with non-adherent membrane (PU-non-ad). NPWT was applied to the wound site. In the control group, basic occlusive dressing (gauze) without NPWT was applied. On days 0, 3, 7, 14, 21 and 28 post-surgery, the wound size was measured during dressing change, and wound healing rate (WHR) was calculated. In addition, blood perfusion within 2cm of the surrounding wound was measured by laser doppler flowmetry. Dressing specimen was collected and microbiology was analysed. Granulation tissues from the central part of the wounds were analysed for histology, vascular endothelial growth factor (VEGF) and cluster of differentiation 31 (CD31) mRNA expression.
UNASSIGNED: The PU-non-ad-NPWT significantly (p<0.01) accelerated wound healing in the pigs. Further pathological analysis revealed that the non-adherent membrane effectively protected granulation tissue formation in PU-NPWT treated wounds. The blood perfusion analysis suggested that the non-adherent membrane improved the blood supply to the wound area. Microbiological analysis showed that non-adherent membrane decreased the bacterial load in the PU-NPWT dressing. VEGF and CD31 mRNA expression was upregulated in the wound tissue from the PU-non-ad-NPWT treated groups.
UNASSIGNED: In this study, the PU dressing with non-adherent membrane was an ideal dressing in NPWT-assisted wound healing.
UNASSIGNED: Excisions were made in the backs of the pigs and were covered with polyvinyl alcohol (PVA) dressing, polyurethane (PU) dressing or PU dressing with non-adherent membrane (PU-non-ad). NPWT was applied to the wound site. In the control group, basic occlusive dressing (gauze) without NPWT was applied. On days 0, 3, 7, 14, 21 and 28 post-surgery, the wound size was measured during dressing change, and wound healing rate (WHR) was calculated. In addition, blood perfusion within 2cm of the surrounding wound was measured by laser doppler flowmetry. Dressing specimen was collected and microbiology was analysed. Granulation tissues from the central part of the wounds were analysed for histology, vascular endothelial growth factor (VEGF) and cluster of differentiation 31 (CD31) mRNA expression.
UNASSIGNED: The PU-non-ad-NPWT significantly (p<0.01) accelerated wound healing in the pigs. Further pathological analysis revealed that the non-adherent membrane effectively protected granulation tissue formation in PU-NPWT treated wounds. The blood perfusion analysis suggested that the non-adherent membrane improved the blood supply to the wound area. Microbiological analysis showed that non-adherent membrane decreased the bacterial load in the PU-NPWT dressing. VEGF and CD31 mRNA expression was upregulated in the wound tissue from the PU-non-ad-NPWT treated groups.
UNASSIGNED: In this study, the PU dressing with non-adherent membrane was an ideal dressing in NPWT-assisted wound healing.
摘要：
■负压伤口治疗（NPWT）被认为是促进各种伤口愈合的有效技术。本研究的目的是评估不同伤口敷料联合NPWT治疗五指山猪伤口的效果。
■在猪的背部进行切除，并用聚乙烯醇（PVA）敷料覆盖，聚氨酯（PU）敷料或具有非粘附膜的PU敷料（PU-non-ad）。将NPWT应用于伤口部位。在对照组中，不使用NPWT的基本闭塞敷料（纱布）。术后第0、3、7、14、21和28天，在换药期间测量伤口大小，并计算伤口愈合率(WHR)。此外,通过激光多普勒血流仪测量周围伤口2cm内的血液灌注。收集敷料标本并分析微生物学。对伤口中央部分的肉芽组织进行组织学分析，血管内皮生长因子（VEGF）和分化簇31（CD31）mRNA表达。
■PU-non-ad-NPWT显著(p<0.01)加速猪的伤口愈合。进一步的病理分析显示,在PU-NPWT处理的伤口中,非粘附膜有效地保护肉芽组织形成。血液灌注分析表明，非粘附膜改善了伤口区域的血液供应。微生物学分析表明，非粘附膜降低了PU-NPWT敷料中的细菌负荷。来自PU-非ad-NPWT处理组的伤口组织中VEGF和CD31mRNA表达上调。
■在这项研究中，具有非粘附膜的PU敷料是NPWT辅助伤口愈合的理想敷料。
■在猪的背部进行切除，并用聚乙烯醇（PVA）敷料覆盖，聚氨酯（PU）敷料或具有非粘附膜的PU敷料（PU-non-ad）。将NPWT应用于伤口部位。在对照组中，不使用NPWT的基本闭塞敷料（纱布）。术后第0、3、7、14、21和28天，在换药期间测量伤口大小，并计算伤口愈合率(WHR)。此外,通过激光多普勒血流仪测量周围伤口2cm内的血液灌注。收集敷料标本并分析微生物学。对伤口中央部分的肉芽组织进行组织学分析，血管内皮生长因子（VEGF）和分化簇31（CD31）mRNA表达。
■PU-non-ad-NPWT显著(p<0.01)加速猪的伤口愈合。进一步的病理分析显示,在PU-NPWT处理的伤口中,非粘附膜有效地保护肉芽组织形成。血液灌注分析表明，非粘附膜改善了伤口区域的血液供应。微生物学分析表明，非粘附膜降低了PU-NPWT敷料中的细菌负荷。来自PU-非ad-NPWT处理组的伤口组织中VEGF和CD31mRNA表达上调。
■在这项研究中，具有非粘附膜的PU敷料是NPWT辅助伤口愈合的理想敷料。
