PDF(Pubmed)
Abstract：
Although human tenocytes and dermal fibroblasts have shown promise in tendon engineering, no tissue engineered medicine has been developed due to the prolonged ex vivo time required to develop an implantable device. Considering that macromolecular crowding has the potential to substantially accelerate the development of functional tissue facsimiles, herein we compared human tenocyte and dermal fibroblast behaviour under standard and macromolecular crowding conditions to inform future studies in tendon engineering. Basic cell function analysis made apparent the innocuousness of macromolecular crowding for both cell types. Gene expression analysis of the without macromolecular crowding groups revealed expression of tendon related molecules in human dermal fibroblasts and tenocytes. Protein electrophoresis and immunocytochemistry analyses showed significantly increased and similar deposition of collagen fibres by macromolecular crowding in the two cell types. Proteomics analysis demonstrated great similarities between human tenocyte and dermal fibroblast cultures, as well as the induction of haemostatic, anti-microbial and tissue-protective proteins by macromolecular crowding in both cell populations. Collectively, these data rationalise the use of either human dermal fibroblasts or tenocytes in combination with macromolecular crowding in tendon engineering.
摘要：
尽管人类肌腱细胞和真皮成纤维细胞在肌腱工程中显示出希望，由于开发可植入装置所需的离体时间较长，因此尚未开发出组织工程药物。考虑到大分子拥挤有可能大大加速功能组织传真的发展，在本文中，我们比较了在标准和大分子拥挤条件下人类肌腱细胞和真皮成纤维细胞的行为，以指导肌腱工程的未来研究。基本的细胞功能分析表明，两种细胞类型的大分子拥挤都是无害的。无大分子拥挤组的基因表达分析显示，人真皮成纤维细胞和肌腱细胞中肌腱相关分子的表达。蛋白质电泳和免疫细胞化学分析显示，两种细胞类型中的大分子拥挤导致胶原纤维的沉积显着增加和相似。蛋白质组学分析表明人类肌腱细胞和真皮成纤维细胞培养物之间有很大的相似性，以及诱导止血，通过两个细胞群体中的大分子拥挤产生抗微生物和组织保护蛋白。总的来说，这些数据使人类真皮成纤维细胞或肌腱细胞与大分子拥挤在肌腱工程中的结合使用合理化。
