Abstract：
BACKGROUND: Enzootic pneumonia is an important disease complex associated with insufficient colostrum intake after birth, adverse environmental conditions, and stress. Vitamin D deficiency may be an important predisposing factor for this disease.
OBJECTIVE: This study aimed to investigate in calves with enzootic pneumonia.
METHODS: A total of 30 calves, aged 3-5 months, under the same care and feeding conditions were used. Groups were formed according to Clinical Respiratory Scoring as the group with mild/moderate enzootic pneumonia (n = 10), the group with severe enzootic pneumonia (n = 10), and the healthy control group (n = 10) without any disease. Blood samples were collected from the jugular vein of animals in all groups on Day 0; a complete blood count was performed, and serum vitamin D levels were measured using the Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) method.
RESULTS: Although no statistical differences were observed in total leukocyte, lymphocyte, eosinophil, basophil, hemoglobin, and hematocrit levels between groups, statistically significant differences in blood neutrophil, monocyte, and erythrocyte counts were found between the groups. Monocyte counts were statistically decreased in the mild/moderate group compared with the control group. Neutrophil counts were significantly higher in the mild/moderate and severe groups than in the control group. Erythrocyte counts were increased in the mild/moderate and severe groups compared with the control group. Vitamin D concentrations were statistically lower in the mild/moderate and severe groups than in the control group. However, no statistical differences in Vitamin D concentrations were observed between the mild/moderate and severe groups. There was a negative and significant correlation between erythrocyte counts and vitamin D concentrations (r = -0.64, P < .0001). While erythrocyte counts increased in the severe group compared with the mild/moderate group, vitamin D concentrations decreased. Also, a negative and significant correlation was observed between platelet counts and vitamin D concentrations (r = -0.74, P < .0001).
CONCLUSIONS: The results of this study determined that serum vitamin D concentrations in calves with pneumonia were lower than those in healthy calves. Detailed studies on the etiologic and prognostic importance of low vitamin D levels in calves with enzootic pneumonia may provide valuable data for prevention and treatment.
OBJECTIVE: This study aimed to investigate in calves with enzootic pneumonia.
METHODS: A total of 30 calves, aged 3-5 months, under the same care and feeding conditions were used. Groups were formed according to Clinical Respiratory Scoring as the group with mild/moderate enzootic pneumonia (n = 10), the group with severe enzootic pneumonia (n = 10), and the healthy control group (n = 10) without any disease. Blood samples were collected from the jugular vein of animals in all groups on Day 0; a complete blood count was performed, and serum vitamin D levels were measured using the Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) method.
RESULTS: Although no statistical differences were observed in total leukocyte, lymphocyte, eosinophil, basophil, hemoglobin, and hematocrit levels between groups, statistically significant differences in blood neutrophil, monocyte, and erythrocyte counts were found between the groups. Monocyte counts were statistically decreased in the mild/moderate group compared with the control group. Neutrophil counts were significantly higher in the mild/moderate and severe groups than in the control group. Erythrocyte counts were increased in the mild/moderate and severe groups compared with the control group. Vitamin D concentrations were statistically lower in the mild/moderate and severe groups than in the control group. However, no statistical differences in Vitamin D concentrations were observed between the mild/moderate and severe groups. There was a negative and significant correlation between erythrocyte counts and vitamin D concentrations (r = -0.64, P < .0001). While erythrocyte counts increased in the severe group compared with the mild/moderate group, vitamin D concentrations decreased. Also, a negative and significant correlation was observed between platelet counts and vitamin D concentrations (r = -0.74, P < .0001).
CONCLUSIONS: The results of this study determined that serum vitamin D concentrations in calves with pneumonia were lower than those in healthy calves. Detailed studies on the etiologic and prognostic importance of low vitamin D levels in calves with enzootic pneumonia may provide valuable data for prevention and treatment.
摘要：
背景：内生性肺炎是一种重要的疾病，与出生后初乳摄入不足有关，不利的环境条件，和压力。维生素D缺乏可能是该疾病的重要诱发因素。
目的：本研究旨在调查小牛患老年肺炎的情况。
方法：总共30头小牛，年龄3-5个月，在相同的护理和喂养条件下使用。根据临床呼吸评分分组为轻度/中度地方性肺炎组（n=10），严重的地方性肺炎组（n=10），健康对照组(n=10)无任何疾病。在第0天从所有组的动物的颈静脉收集血样;进行全血细胞计数,使用液相色谱-串联质谱(LC-MS/MS)方法测量和血清维生素D水平。
结果：尽管没有观察到总白细胞的统计学差异，淋巴细胞，嗜酸性粒细胞,嗜碱性粒细胞,血红蛋白,和组间血细胞比容水平，血液中性粒细胞的统计学差异，单核细胞,各组之间发现红细胞计数。与对照组相比，轻度/中度组的单核细胞计数在统计学上降低。轻度/中度和重度组的中性粒细胞计数明显高于对照组。与对照组相比，轻度/中度和重度组的红细胞计数增加。轻度/中度和重度组的维生素D浓度在统计学上低于对照组。然而,轻度/中度和重度组之间的维生素D浓度无统计学差异.红细胞计数与维生素D浓度呈负相关（r=-0.64，P<0.0001）。与轻度/中度组相比，重度组红细胞计数增加，维生素D浓度下降。此外，血小板计数与维生素D浓度呈负相关(r=-0.74,P<0.0001).
结论：这项研究的结果确定，肺炎小牛的血清维生素D浓度低于健康小牛。详细研究维生素D水平低对小牛地方性肺炎的病因学和预后的重要性，可能为预防和治疗提供有价值的数据。
目的：本研究旨在调查小牛患老年肺炎的情况。
方法：总共30头小牛，年龄3-5个月，在相同的护理和喂养条件下使用。根据临床呼吸评分分组为轻度/中度地方性肺炎组（n=10），严重的地方性肺炎组（n=10），健康对照组(n=10)无任何疾病。在第0天从所有组的动物的颈静脉收集血样;进行全血细胞计数,使用液相色谱-串联质谱(LC-MS/MS)方法测量和血清维生素D水平。
结果：尽管没有观察到总白细胞的统计学差异，淋巴细胞，嗜酸性粒细胞,嗜碱性粒细胞,血红蛋白,和组间血细胞比容水平，血液中性粒细胞的统计学差异，单核细胞,各组之间发现红细胞计数。与对照组相比，轻度/中度组的单核细胞计数在统计学上降低。轻度/中度和重度组的中性粒细胞计数明显高于对照组。与对照组相比，轻度/中度和重度组的红细胞计数增加。轻度/中度和重度组的维生素D浓度在统计学上低于对照组。然而,轻度/中度和重度组之间的维生素D浓度无统计学差异.红细胞计数与维生素D浓度呈负相关（r=-0.64，P<0.0001）。与轻度/中度组相比，重度组红细胞计数增加，维生素D浓度下降。此外，血小板计数与维生素D浓度呈负相关(r=-0.74,P<0.0001).
结论：这项研究的结果确定，肺炎小牛的血清维生素D浓度低于健康小牛。详细研究维生素D水平低对小牛地方性肺炎的病因学和预后的重要性，可能为预防和治疗提供有价值的数据。
