PDF(Pubmed)
Abstract：
Eukaryotic RNA polymerase II (RNAPII) is responsible for the transcription of the protein-coding genes in the cell. Enormous progress has been made in discovering the protein activities that are required for transcription to occur, but the effects of post-translational modifications (PTMs) on RNAPII transcriptional regulation are much less understood. Most of our understanding relates to the cyclin-dependent kinases (CDKs), which appear to act relatively early in transcription. However, it is becoming apparent that other PTMs play a crucial role in the transcriptional cycle, and it is doubtful that any sort of complete understanding of this regulation is attainable without understanding the spectra of PTMs that occur on the transcriptional machinery. Among these is O-GlcNAcylation. Recent experiments have shown that the O-GlcNAc PTM likely has a prominent role in transcription. This review will cover the role of the O-GlcNAcylation in RNAPII transcription during initiation, pausing, and elongation, which will hopefully be of interest to both O-GlcNAc and RNAPII transcription researchers.
摘要：
真核RNA聚合酶II（RNAPII）负责细胞中蛋白质编码基因的转录。在发现转录发生所需的蛋白质活性方面取得了巨大进展，但是翻译后修饰（PTM）对RNAPII转录调控的影响却知之甚少。我们的大部分理解与细胞周期蛋白依赖性激酶(CDK)有关，似乎在转录中相对较早地起作用。然而,很明显，其他PTM在转录周期中起着至关重要的作用，如果不了解转录机制上发生的PTM的光谱，那么对这种调节的任何完全理解都是值得怀疑的。其中包括O-GlcNAcylation。最近的实验表明O-GlcNAcPTM可能在转录中具有突出的作用。这篇综述将涵盖O-GlcNAcylation在RNAPII转录起始过程中的作用，暂停,和伸长率，O-GlcNAc和RNAPII转录研究人员都希望对此感兴趣。
