Abstract：
OBJECTIVE: The causes of idiopathic infertility are still not known; however, it may be associated with microbial etiologies. The present study examines the vaginal microbiota of infertile as well as fertile women longitudinally.
METHODS: The study was presented and accepted by the Institutional Ethical Committee of Shri Mata Vaishno Devi University, Katra, Jammu and Kashmir (India). An observational, prospective, multicenteric investigation was conducted at the Department of Obstetrics and Gynecology, Government Medical College Jammu, and its affiliated hospitals in Jammu and Kashmir (India). In order to examine the microbial composition, a cohort of 80 female individuals were involved in the screening process. The investigation involved sequencing of the V3-V4 region of 16S rRNA gene, which was subsequently analyzed using the Mothur pipeline.
RESULTS: The study revealed that the vaginal microbiota of infertile women differed from that of healthy women who had previously given birth without any complications. Both populations have variations in their alpha as well as beta diversity and taxonomical composition. The microbial profiles in the cases of infertility are characterized by elevated levels of Gardnerella, Prevotella, Atopobium, and Enterococcus whereas a higher level of Lactobacillus iners was observed in case of fertile women.
CONCLUSIONS: In conclusion, it can be inferred that the composition of the vaginal microbiome potentially exerts a significant influence on females afflicted with idiopathic infertility.
METHODS: The study was presented and accepted by the Institutional Ethical Committee of Shri Mata Vaishno Devi University, Katra, Jammu and Kashmir (India). An observational, prospective, multicenteric investigation was conducted at the Department of Obstetrics and Gynecology, Government Medical College Jammu, and its affiliated hospitals in Jammu and Kashmir (India). In order to examine the microbial composition, a cohort of 80 female individuals were involved in the screening process. The investigation involved sequencing of the V3-V4 region of 16S rRNA gene, which was subsequently analyzed using the Mothur pipeline.
RESULTS: The study revealed that the vaginal microbiota of infertile women differed from that of healthy women who had previously given birth without any complications. Both populations have variations in their alpha as well as beta diversity and taxonomical composition. The microbial profiles in the cases of infertility are characterized by elevated levels of Gardnerella, Prevotella, Atopobium, and Enterococcus whereas a higher level of Lactobacillus iners was observed in case of fertile women.
CONCLUSIONS: In conclusion, it can be inferred that the composition of the vaginal microbiome potentially exerts a significant influence on females afflicted with idiopathic infertility.
摘要：
目的：特发性不孕症的病因尚不清楚；它可能与微生物病因有关。本研究纵向检查了不育和可育妇女的阴道微生物群。
方法：该研究由ShriMataVaishnoDevi大学的机构伦理委员会提出并接受，Katra,查谟和克什米尔（印度）。一个观察,prospective,在妇产科进行了多中心调查，政府医学院查谟,及其在查谟和克什米尔（印度）的附属医院。为了检查微生物组成，一个由80名女性组成的队列参与了筛查过程.这项调查涉及16SrRNA基因V3-V4区的测序，随后使用莫瑟管道进行了分析。
结果：研究表明，不孕妇女的阴道微生物群与健康妇女的阴道微生物群不同，健康妇女先前分娩时没有任何并发症。两个种群的α和β多样性和分类学组成都存在差异。不孕症的微生物特征是加德纳菌水平升高，普雷沃氏菌,Atobobium,和肠球菌，而在有生育能力的妇女中观察到较高水平的乳酸杆菌。
结论：结论：可以推断，阴道微生物组的组成可能对患有特发性不育症的女性产生重大影响。
方法：该研究由ShriMataVaishnoDevi大学的机构伦理委员会提出并接受，Katra,查谟和克什米尔（印度）。一个观察,prospective,在妇产科进行了多中心调查，政府医学院查谟,及其在查谟和克什米尔（印度）的附属医院。为了检查微生物组成，一个由80名女性组成的队列参与了筛查过程.这项调查涉及16SrRNA基因V3-V4区的测序，随后使用莫瑟管道进行了分析。
结果：研究表明，不孕妇女的阴道微生物群与健康妇女的阴道微生物群不同，健康妇女先前分娩时没有任何并发症。两个种群的α和β多样性和分类学组成都存在差异。不孕症的微生物特征是加德纳菌水平升高，普雷沃氏菌,Atobobium,和肠球菌，而在有生育能力的妇女中观察到较高水平的乳酸杆菌。
结论：结论：可以推断，阴道微生物组的组成可能对患有特发性不育症的女性产生重大影响。
