Abstract：
This study aimed to investigate the genetic diversity of 108 geographically and temporally diverse strains of Mycoplasma hominis using a multi-locus sequence typing scheme (MLST). We extracted MLST data of 87 strains from PubMLST database and retrieved MLST gene sequences from 21 complete genomes of M. hominis available in GenBank database. MLST scheme identified 65 Sequence types (STs), which were grouped into five clonal complexes (CC) and 47 singletons. Phylogenetic analysis revealed that the majority of M. hominis isolates were clustered according to their country of origin, showing some significant specificity trends for the nation. Although recombination was detected, it was not significant enough to alter the clonal population structure of M. hominis. In sum, MLST scheme provides insightful data on the phylogenetics of international strains of M. hominis, arguing for the existence of genetically differentiable STs according to their origin of isolation.
摘要：
本研究旨在使用多位点序列分型方案（MLST）研究108个地理和时间上不同的人型支原体菌株的遗传多样性。我们从PubMLST数据库中提取了87个菌株的MLST数据，并从GenBank数据库中提供的21个完整的人型支原体基因组中检索了MLST基因序列。MLST方案确定了65种序列类型(ST)，分为5个克隆复合物（CC）和47个单例。系统发育分析表明，大多数人源分离株根据其起源国进行聚类，显示出国家的一些显著的特异性趋势。尽管检测到重组，这并不足以改变人源的克隆种群结构。总之,MLST方案提供了有关人源M.hominis国际菌株的系统发育学的有见地的数据，根据分离的起源，争论遗传上可分化的STs的存在。
