Abstract：
This study investigated the prevalence and associated risk factors of Campylobacter in South Korean broilers using a random-effects meta-analysis. Subsequently, to facilitate the design of preventive measures, the prevalence estimate from the meta-analysis was incorporated into a stochastic risk assessment model to quantify the Campylobacter contamination levels on broiler carcasses. The baseline model was developed based on the most common practices along the South Korean broiler processing line, with no interventions. Meta-analysis results revealed Campylobacter prevalence across the chicken supply chain in the following order: farms (60.6 % [57.3-63.4]), retail markets (43.90 % [24.81-64.99]), slaughterhouses (27.71 % [18.56-39.21]), and processing plants (14.50 % [3.96-41.09]). The model estimated a 52 % (36.1-70.8) Campylobacter prevalence at the end of chilling, with an average contamination level of 4.62 (2.50-6.74) log CFU/carcass. Sensitivity analysis indicated that Campylobacter fecal shedding (r = 0.95) and the amount of feces on bird exteriors (r = 0.17) at pre-harvest were the main factors for carcass contamination, while soft scalding (r = -0.22) and air chilling (r = -0.12) can serve as critical control points (CCPs) at harvest. Scenario analysis indicated that a combination of hard scalding, inside-outside bird washing, spray washing, and chlorinated water immersion chilling can offer a 30.9 % reduction in prevalence and a reduction of 2.23 log CFU/carcass in contamination levels compared to the baseline model. Apart from disinfection and sanitation interventions carried out during meat processing, the implementation of robust control measures is indispensable to mitigate Campylobacter prevalence and concentration at broiler farms, thereby enhancing meat safety and public health. Furthermore, given the high Campylobacter prevalence in the retail markets, future studies should explore the potential risk of cross-contamination at post-harvest stage.
摘要：
这项研究使用随机效应荟萃分析调查了韩国肉鸡中弯曲杆菌的患病率和相关危险因素。随后，为了便于预防措施的设计，将荟萃分析的患病率估计值纳入随机风险评估模型,以量化肉鸡屠体中弯曲杆菌的污染水平.基线模型是根据韩国肉鸡加工线上最常见的做法开发的，没有干预。荟萃分析结果显示，整个鸡肉供应链中弯曲杆菌的流行顺序如下：农场（60.6%[57.3-63.4]），零售市场（43.90%[24.81-64.99]），屠宰场（27.71%[18.56-39.21]），和加工厂（14.50%[3.96-41.09]）。该模型估计在冷却结束时弯曲杆菌的患病率为52％（36.1-70.8），平均污染水平为4.62(2.50-6.74)对数CFU/car体。敏感性分析表明，收获前弯曲杆菌粪便脱落（r=0.95）和鸟类外部粪便数量（r=0.17）是car体污染的主要因素，而软烫伤（r=-0.22）和空气冷却（r=-0.12）可以作为收获时的关键控制点（CCP）。情景分析表明，硬烫伤的组合，内外洗鸟，喷雾洗涤,与基线模型相比，氯化水浸冷可以使患病率降低30.9％，污染水平降低2.23logCFU/car体。除了在肉类加工过程中进行消毒和卫生干预外，实施强有力的控制措施对于减轻肉鸡养殖场弯曲杆菌的流行和浓度是必不可少的，从而提高肉类安全和公众健康。此外，鉴于零售市场中弯曲杆菌的高流行率，未来的研究应探讨收获后交叉污染的潜在风险.
